FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876739
Sequences flagged as poor quality0
Sequence length35-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC14190.16184976372671914No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC12630.14405655502948997No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT11090.12649146439248168No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA10970.12512275603115636No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA10630.12124474900740129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATA2500.069.3612594-95
CTCGTCA3050.063.6690641
CCGAATA3350.063.4594232
CCCGAAT3350.062.208871
CGAATAG2700.059.4899443
TCGTCAT3400.056.968312
CGGCGCA602.4525434E-855.1188285
GTCTATC6400.054.6266945
CGTCATT3450.053.404023
ACGGCGC558.498664E-751.539694
CGAATAC1150.049.2960173
GTGAGTT10700.046.8031736
TCTATCG652.6864527E-643.61056
TGAGTTG12050.043.130417
CAGTCTA8000.043.108343
AGTCTAT8150.042.8970384
GATCGCC1900.042.2691732
CGTCGGA459.882457E-441.9952964
CCCGTCG459.885231E-441.9929052
TGTGAGT11950.041.9074445
Produced by FastQC (version 0.11.5)