FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719014
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC14820.2061155971928224No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC12590.17510090206866627No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA11710.16286191923940285No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT11520.16021941158308461No Hit
ACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGC10350.14394712759417758No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA8920.12405878049662454No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG8040.11181979766736114No Hit
GCTGATAAGCAACTTCAGCAAAGTCTCAGGATACAAAATCAATGTACAAA7420.10319687794674373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA2200.081.5860752
AACGGCG255.4228138E-575.574463
CGAATAG3000.064.553193
CGGCGCA301.3375394E-462.9787185
ACGGCGC301.3375394E-462.9787184
CCCGAAT2900.062.0741841
GATCGCC1150.061.6096232
CGTCATT2050.057.602493
GTCTATC6300.056.2315
TGATCGC1350.056.1450731
TATGCCG352.8656094E-453.9817626
AGTCTAT6300.053.981764
GTGAGTT11850.052.216536
TCGCCAT1550.051.8050774
CGAATAC558.509742E-751.5280463
TGAGTTG12500.051.401367
GATAAGC5550.048.5106354
TCGTCAT2450.048.1982
GAGTTGA14400.047.9000638
GCCGCCG502.9176748E-547.2438969
Produced by FastQC (version 0.11.5)