FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068849
Sequences flagged as poor quality0
Sequence length35-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC26820.250924124923165No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT23690.22164028782363085No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC17400.16279193786961488No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA16310.1525940521065183No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA14360.13435012803492355No Hit
TGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATT13650.1277074685011634No Hit
ACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGC12800.11975498877764773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGA201.7908218E-594.491954
CCGAATA5150.077.06142
CCCGTCG255.4172324E-575.593572
CCCGAAT5150.075.410051
CGAATAG3950.074.158243
GATCGCC2250.073.4937442
TGATCGC2450.071.525061
AACGGCG301.3361644E-462.9946373
ACGGCGC301.3361644E-462.9946374
GTCTATC10900.062.4167025
CGCCATT2850.058.0213785
AGTCTAT12800.056.1045954
CGAATAC1950.055.7260253
GTGAGTT17900.055.1642956
TCGCCAT2950.054.452994
CGTCATT4100.054.1600233
CCGTCGG352.8626682E-453.99543
CAGTCTA13450.053.3932233
TCGTCAT4250.053.3601652
CTCGTCA4200.052.9994241
Produced by FastQC (version 0.11.5)