FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005299799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305034
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT48650.37278722240186846No Hit
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC47350.36282579610952664No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA29190.22367233344112109No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC27330.2094198312074628No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG22600.1731755647745576No Hit
TTCCATTCCATTCCATTCGATTGCAATCGAGTTGATTCCATTCCATTCCA18100.13869370453183594No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA17930.13739105647822203No Hit
TGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATT15160.11616555583992448No Hit
ATAGGGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATC14720.11279399617174725No Hit
CCCGAATAGAATGGAATGGAATGGAATGGAACGGAACGGAATGGAATGGA13760.1054378659866333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)