FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340120
Sequences flagged as poor quality0
Sequence length35-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC21590.16110497567382026No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT18240.13610721427931827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGA255.4173674E-575.59464
CGTCATT4550.068.533563
GATCGCC2400.066.9327242
CCGAATA2950.065.66482
TCGTCAT5100.062.06912
CTCGTCA5000.059.7022671
TGATCGC2800.059.2284431
CGAATAG3000.058.2708363
CCCGAAT3550.056.0584641
CGCCATT3400.050.0258375
GTCTATC9700.046.272475
TGCGGCG601.8189894E-1243.3255766-67
TCGCCAT3950.043.0602154
GATTGCC8350.043.0029142
CCGTCGG459.882362E-441.9969983
CCCGTCG459.882362E-441.9969982
GCCATTC15100.040.363016
GCGCACC608.54941E-539.3780677
GGGAATC9850.038.3728944
CGGCGCA500.001658930137.79735
Produced by FastQC (version 0.11.5)