FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890582
Sequences flagged as poor quality0
Sequence length35-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC14040.15764971670211167No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT12440.13968393702095933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA2100.074.232772
CTCGTCA3300.060.295061
CGTCATT3350.056.404823
TGATCGC1850.056.3374671
CGAATAG2200.053.6807253
TCGTCAT3750.052.9077262
CCCGAAT3050.052.811131
GATCGCC1950.050.8728072
GTCTATC6250.050.640255
TCGCGCT405.5358466E-447.2390336
GTGAGTT12250.044.346856
GGGAATC6850.044.1357424
CTTCGCG459.888384E-441.9902574
GGAATCC7050.041.5435525
ATAGGGA7450.041.3336221
TTGAACG5300.041.0091310-11
TGAACGC5150.040.3686312-13
CAGTCTA7850.040.318673
TGAGTTG12900.040.2903987
AGTCTAT8250.040.0816084
Produced by FastQC (version 0.11.5)