FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963787
Sequences flagged as poor quality0
Sequence length35-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT25460.2641662525018495No Hit
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC25450.2640624951363735No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG11200.11620824933309952No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC10870.11278425627239212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCA5150.068.089251
CGTCATT5300.067.749423
GTCTATC11300.062.314395
CAGTCTA12350.060.444663
AGTCTAT12100.058.975564
TCGTCAT6200.057.9148182
CTATCAT12800.053.537877
TGATCGC2150.050.6924631
ATCATTG13850.049.1378069
TCTATCA13950.048.783036
TCCAGTC15200.048.321691
CCAGTCT15950.046.5057642
TATCATT14900.045.675088
GATCGCC2400.041.3405152
ATAGGGA7050.041.0010261
CGAGTAT704.4709977E-640.496832
CCGCAAT4150.039.87938360-61
GATTGCC5450.039.8776132
GTGAGTT12750.039.2894746
TGTGAGT13050.038.7483985
Produced by FastQC (version 0.11.5)