FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214871
Sequences flagged as poor quality0
Sequence length35-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC23300.19178991020445793No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT16360.1346645034740314No Hit
ACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGC15180.12495153806453525No Hit
TGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATT14610.1202596818921515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCA4150.063.9867481
GATCGCC3000.062.9960142
TGATCGC3100.062.7151221
CCGAATA3100.059.4397852
CGTCATT4800.057.0901373
CGAATAG3200.056.105823
CCCGAAT3200.054.827931
TCGCCAT4000.053.152884
TCGTCAT5300.052.5957262
CGCCATT4100.051.8564725
GTCTATC8800.045.636315
ATAGGGA9800.043.5478321
GGGAATC9800.043.390114
AGTCAGG16100.043.1385733
GCCATTC13800.042.453836
CAGTCTA9850.042.210523
GATTGCC7350.041.7830662
TGGAACC10000.040.161619
GGAATCC10600.039.223935
ATCGCCA5450.039.0112883
Produced by FastQC (version 0.11.5)