FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279972
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA40950.3199288734441066No Hit
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC39460.3082879937998644No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT36790.2874281624910545No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC35800.27969361829790024No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG17530.136956120915145No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA16670.13023722393927367No Hit
TTCCATTCCATTCCATTCGATTGCAATCGAGTTGATTCCATTCCATTCCA15510.12117452569274953No Hit
TGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATT12900.10078345463807022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT9300.082.030751
CCGAATA10100.081.857632
CGAATAG7700.081.6024863
GATCGCC2550.070.4021452
GTCTATC14500.070.382095
CGAATAC3300.068.717883
TGATCGC2800.064.307161
AGTCTAT16200.063.579624
CGTCATT5650.060.2041633
CTCGTCA5950.059.7280121
CAGTCTA17550.058.6842843
AACGGCG657.039489E-1058.14593
GTGAGTT23150.056.9415936
TTCATAG21550.055.9095889
CGCGAAT352.821866E-454.1533971
TGTGAGT24700.053.559625
TCGTCAT6450.053.4694372
TGAGTTG25150.053.166967
CGCCATT3850.050.315245
ACGGCGC851.364242E-1050.026484
Produced by FastQC (version 0.11.5)