FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213661
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC25350.2088721644676726No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT23470.19338184221129293No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA13950.11494148695558315No Hit
TGATTGCCATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATT13090.10785548847660097No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA13020.10727872115854427No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC12900.10628997718473279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA4550.071.64692
CCCGAAT4650.069.295621
ACGGCGC659.094947E-1265.419424
CGAATAG3350.064.87433
GTCTATC9800.063.639315
AACGGCG657.039489E-1058.1482053
CGGCGCA754.0017767E-1156.6968355
GATCGCC2500.056.69452
TCGTCAT5150.055.960592
CGTCATT5150.055.960593
CTCGTCA5000.054.9677351
CGAATAC2250.054.5947073
TGATCGC2700.054.4061241
GGCGCAC1150.053.410066
CAGTCTA13000.049.062553
GCGCACC1350.049.00137
AGTCTAT13300.047.6026084
TCTATCG1250.045.357476
GATTGCC7650.045.083862
GTGAGTT13400.044.074036
Produced by FastQC (version 0.11.5)