FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516466
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC33120.2184025227074No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT30640.202048710620614No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC25170.16597800412274327No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA21790.14368934087543012No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG16120.10629977856410892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA6050.069.498022
CGAATAG5350.066.228553
CCCGAAT5950.066.080711
CACGATA3200.060.79239794-95
GTCTATC13600.060.095925
CGTCATT7000.060.0661433
TCGTCAT7100.059.2201462
CTCGTCA7600.056.097371
TGATCGC2900.055.5385441
AGTCTAT15150.055.194834
CAGTCTA15900.052.59133
GATCGCC3200.051.6720732
CGAATAC1750.048.5928343
TTCATAG21950.048.2179349
TCGTCGA404.4074113E-947.2773366-67
AACGGCG502.9195335E-547.2430343
ACGGCGC601.5494406E-647.2430344
GTGAGTT23700.046.6450236
GATTGCC8950.046.451252
GCGCTTG2300.045.188992
Produced by FastQC (version 0.11.5)