FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956607
Sequences flagged as poor quality0
Sequence length35-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAG4300.063.737533
CCCGAAT4750.059.867231
CCGAATA4900.059.7903752
CGGCCGC652.6832022E-643.6187863
TAAGCGG1006.693881E-1042.5283169
TCGGCCG608.5475665E-539.378072
TTCATAG12200.039.1198549
CTCGTCA2350.036.3024671
ACGAGTA1107.338531E-834.3663183
GCAACTT7150.034.3663149
TATCGAG1255.9444574E-934.022658
TCGTCAT2550.033.3555452
TCGTTGG7600.033.26410778-79
CGGGGGT1308.714778E-932.7140923
ACTGTCG1456.8030204E-1032.686131
CGATAAG1900.032.331477
TGAGTTG10550.031.801067
CGCTTGG1501.0095391E-931.5024576
GCTGATA8150.031.4027461
CTTCGTT7600.031.40211576-77
Produced by FastQC (version 0.11.5)