FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005299811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005299811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450683
Sequences flagged as poor quality0
Sequence length35-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAACAC43040.2966878360055229No Hit
ACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCATTC34610.23857727704812148No Hit
CCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCTGCA33610.23168397230821622No Hit
TCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCTATT31040.21396817912665966No Hit
TTCCATTCCATTCCATTCGATTGCAATCGAGTTGATTCCATTCCATTCCA22550.1554440218848639No Hit
CCCGAATAGAATGGAATGGAATGGAATGGAACGGAACGGAATGGAATGGA21710.14965364590334346No Hit
GAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAAATG21370.14730992229177567No Hit
ACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGC15440.10643262518413742No Hit
AGTAGTTTTTTCCAATTCTGTGAAGAAAGTCATTGGTAGCTTGATGGGGA15360.10588116080494497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA19050.085.5514142
CCCGAAT18050.085.073681
CGAATAG17050.080.071233
CGTCGGA200.002119099570.866214
AACGGCG603.6379788E-1270.858883
GATCGCC2000.068.496922
ACGGCGC659.094947E-1265.414964
CGAATAC5150.065.125973
GTCTATC13200.064.065925
CGTCATT5650.063.5430683
CGCGAAT301.3157407E-463.192591
CTCGTCA5500.061.181921
CGGCGCA702.0008883E-1160.7424665
TGATCGC2550.057.616771
AGTCTAT15250.056.692974
CAGTCTA15550.054.9858863
TCGTCAT6550.054.090752
GTGAGTT21700.050.5098656
CGCCATT3200.050.1968965
AGTCAGG22050.049.488743
Produced by FastQC (version 0.11.5)