FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41710
Sequences flagged as poor quality0
Sequence length199-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1525536.573963078398464No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA993023.807240469911292No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA445910.690481898825222No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA434410.414768640613762No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA11142.670822344761448No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAACGCA10642.5509470151042914No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA5471.3114361064492928No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA3960.94941261088468No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA3800.9110525053943898No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA3750.8990649724286741No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA2010.48189882522176936No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA1660.39798609446175975No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1640.39319108127547353No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1430.3428434428194677No Hit
TACGTAGGTGGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGGGAACGCA1350.3236633900743227No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA1280.3068808439223208No Hit
TACGGAAGGTCCTGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1240.2972908175497483No Hit
TACGTAGGTGGCAAGCGTTGTCCAGATTTATTGGGCGTAAAGGGAACGCA1110.26612323183888753No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1090.2613282186526013No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA960.2301606329417406No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA790.18940302085830737No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA710.17022296811316232No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA680.1630304483337329No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA660.15823543514744665No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA630.15104291536801726No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA550.13186286262287222No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA530.12706784943658594No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA490.11747782306401341No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA450.1078877966914409No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA420.1006952769120115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCAGG151.8232822E-7736.43634195
AAGGCGG2650.0694.7512195
AAGGCAG1750.0631.2312195
AAGCGGG550.0602.5388195
GAAGGCA5950.0532.2145195
TGGCAAG14300.0194.26389
GTCACGA204.9085975E-7194.26388
GGTCCTG100.0029463128194.26388
GGTCCCG20400.0194.26387
GGTCCAG550.0194.26388
TACGGAG100.0029463128194.26381
AGGTGGC14300.0194.26386
CGGAAGG1100.0194.26383
GTCCCGA20400.0194.26388
GTCCTGG100.0029463128194.26389
TCACGAG204.9085975E-7194.26389
AGGTCCT100.0029463128194.26387
AGGTCCG400.0194.26387
AGGTCCC20400.0194.26386
AGGTCCA550.0194.26387
Produced by FastQC (version 0.11.5)