FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268395
Sequences flagged as poor quality0
Sequence length193-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3281912.227873097486913No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG215658.034799456025633No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA204627.623837999962742No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC175266.529927904767227No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG115174.2910635444028395No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA107123.991132472661562No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC102173.8067028074293483No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG96503.595447009072449No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA86783.2332942118891936No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG86353.2172730490508394No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG82303.066376050224483No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG78272.9162242217627004No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA70272.6181560759328604No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG66242.468004247471078No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA62842.341325285493396No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA53621.9978017474245051No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG52901.9709756142998192No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA46521.7332662680005217No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG43951.6375118761526852No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC41771.556288306414054No Hit
CCTGTTCGATCCCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC39971.48922297360234No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA33111.2336295385532519No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA29971.1166377913150394No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAAATTGTTAGTA27421.0216285698317777No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA23600.879301030198029No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA21720.8092550159280166No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA18520.6900277575960804No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA17730.6605935281953836No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGCCTTAGTA15310.570427914081857No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA14880.554406751243503No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC12760.4754186925985953No Hit
CCTGTTTGCTACCCACGCCTTCGAGCCTCAACGTCAGTTATCTTCCAGTA12300.4582797742133795No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG12100.4508280705676335No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA10650.396803219135975No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCCAGCA10230.38115464147990835No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA9180.3420331973397418No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG8690.3237765234076641No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA6440.2399448573930215No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA6130.22839471674211517No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC5760.21460906499748506No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT5740.21386389463291045No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA5550.20678477616945176No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA5360.19970565770599302No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG5210.19411687997168353No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA4780.17809571713332958No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCTAGCA4690.1747424504927439No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA4460.166172991300136No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATTGGCCAGTA3930.14642597663890908No Hit
CCTGTTCGATACCCACGCTTTCGAGCATCAGCGTCAGTTGCGCTACAGTA3800.14158236926917417No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG3490.13003222861826785No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG3310.12332569533709645No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA3040.11326589541533934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGG500.0304.99738195
GTCACCG7200.0304.99738195
TATGCCG1200.0304.99738195
AGGGCCG107.655194E-4304.99738195
TCAACCG107.655194E-4304.99738195
CTGGCCG22400.0304.99738195
TAATCCG3050.0304.99738195
CAAACGG600.0304.99738195
GGAACCG850.0304.99734195
ATATCCG9100.0303.32156195
CCAACCG8050.0303.10297195
CCAGCCG7750.0303.02963195
GTAACCG11800.0302.41266195
ATCGCCG4250.0301.40915195
TCGTCCG3700.0300.8758195
TAGGCCG6950.0300.60892195
GCAACCG6900.0298.367195
ATTGCCG2100.0297.7355195
CAAGCCG5800.0283.96307195
ATTTCCG550.0277.27036195
Produced by FastQC (version 0.11.5)