FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86758
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1979022.810576546255103No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1092412.591346043016205No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA1068212.312409230272712No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA77598.943267479656054No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA55676.416699324557966No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA50075.771225708292031No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA49175.667488877106433No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA41234.752299499757948No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA40394.655478457318058No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA26883.0982733580764887No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA24012.767468129740197No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA18032.0781945180847874No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA9291.0707946241268818No Hit
TACGCAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2170.2501210263030499No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA1590.18326840176122086No Hit
TACGCAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA1540.17750524447313218No Hit
TACGCAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1250.14407893220221765No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA1120.12909472325318702No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGAGCGCA1110.12794209179556928No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA930.10719472555844994No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA900.10373683118559672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCAG100.002327031210.32213195
AAGGCAG33800.0208.7665195
AAGGCGG32850.0207.44101195
AAGGCGA12850.0205.4119195
CGGAGGT153.7702575E-5194.926823
AGGGCGC153.7702575E-5194.926826
TACGTAG53800.0194.926821
CGGAGGG4650.0194.92683
CGGAGGA7350.0194.92683
GAGGATC7350.0194.92685
TACAGAG18700.0194.92681
AGGATCC7350.0194.92686
GTCGCAA100.0029218462194.92688
GGTCCCG6050.0194.92687
ACGTAGT100.0029218462194.92682
CGAAGGG1050.0194.92683
CAGAGGG18650.0194.92683
GTCCCGA6050.0194.92688
CAGGGTG256.2864274E-9194.92685
TACGCAG800.0194.92681
Produced by FastQC (version 0.11.5)