FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005301180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86758
Sequences flagged as poor quality0
Sequence length198-201
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1723519.86560317204177No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT1035011.929735586343622No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1020611.763756656446668No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG75428.693146453353005No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG52796.084741464764056No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG47685.495746789921391No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG42504.898683694875401No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC40724.693515295419442No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA36744.234767975287582No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG28073.2354365015329996No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA24362.807810230756818No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA15641.8027155997141473No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT9391.082320938703059No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG5710.6581525622997303No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA3730.4299315336914175No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA2410.2777841812858757No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1910.2201526084049886No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG1760.20286313654072247No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGGA1670.1924894534221628No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGAA1350.15560524677839507No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTTGCA1320.15214735240554186No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG1300.14984208949030636No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAA1280.1475368265750709No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT1110.12794209179556928No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG1080.12448419742271605No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGCTTGGCA1070.12333156596509831No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGCGTCAGTATTAGGCCAGAT1020.1175684086770096No Hit
CCTGTTCGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAC920.1060420941008322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)