FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119661
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4820340.2829660457459No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2248518.79058339809963No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1611213.464704456757005No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1403111.72562489031514No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA37223.1104536983645468No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA24732.066671680831683No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA18371.5351701891175904No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA16981.419008699576303No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA15401.2869690208171418No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA5380.4496034631166378No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA4850.4053116721404635No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA4720.3944476479387603No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA2650.2214589548808718No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA1920.16045328051746183No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA1750.14624647963831156No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1620.13538245543660843No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGAGCGTA1430.11950426621873458No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1390.11616148954128748No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1220.10195468866213721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGG1500.0715.2154195
ACGGCAG105.9310896E-5715.2154195
AAGATAG2650.0715.21533195
AAGCCGG152.0867992E-7715.21533195
AAGGCAG18050.0711.253195
AAGGCGG6750.0641.0449195
GTCGCGA204.9459595E-7194.27088
TGTCCCG100.0029528625194.27087
GGTCCCG68100.0194.27087
TATGTCC100.0029528625194.27085
TACGGAG750.0194.27081
TCGCGAG204.9459595E-7194.27089
AGGGCCA100.0029528625194.27087
CCGTAGG100.0029528625194.27082
GGCCAGG100.0029528625194.27089
ATGTCCC100.0029528625194.27086
AGGTCCC68100.0194.27086
AGGTCCA3600.0194.27087
GTAGGTC68100.0194.27084
GTAGGGT256.4301275E-9194.27084
Produced by FastQC (version 0.11.5)