FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104449
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4431942.43123438233013No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1704616.31992647129221No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1620015.50996179953853No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA74577.1393694530344955No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA38713.7061149460502256No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA35913.438041532231041No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA27392.622332430181237No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA21192.028741299581614No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA7850.7515629637430707No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA7070.6768853698934407No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA5010.4796599297264694No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2820.26998822391789296No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1480.14169594730442608No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1470.14073854225507185No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1190.11393120087315341No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1110.10627196047831956No Hit
TACGTAGGTCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1080.10339974533025686No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1060.1014849352315484No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1050.10052753018219417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCA104.568364E-5779.9846195
AAGGCAG8400.0770.6991195
AAGCGGG1700.0757.04395195
AAGGCGG3350.0733.4184195
GAAGGCA15400.0357.07086195
GAGGGCA851.1186785E-9229.40726195
GTCGCGA351.8189894E-12194.249058
TCGCGAG351.8189894E-12194.249059
GTCCCGA63800.0194.249058
TAGGGCG8500.0194.249055
GAGGTCC204.945723E-7194.249045
TGGCAAG21100.0194.249049
ACAGAGG153.8252103E-5194.249042
TACAGAG153.8252103E-5194.249041
TGCCGAG100.0029533154194.249049
GGTCCGG3800.0194.249048
GTCCGGG3800.0194.249049
GGTCCCG63850.0194.249047
GGTCTCG100.0029533154194.249047
GGTCCAG3950.0194.249048
Produced by FastQC (version 0.11.5)