FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104449
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3710535.52451435628872No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1368713.104002910511351No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1313512.575515323267815No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG71706.864594203869831No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG48494.6424570843186626No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC34053.2599641930511543No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC33213.1795421689053986No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG24062.3035165487462783No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA18401.7616252908117838No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA17291.655353330333464No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA13481.2905820065295024No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG9010.8626219494681614No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG8590.8224109373952838No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA4070.3896638550871717No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2480.2374364522398491No Hit
CCTGTTCGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1830.17520512403182414No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG1520.145525567501843No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG1170.11201639077444495No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA1080.10339974533025686No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1080.10339974533025686No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGCGTTCCAGCA1070.10244234028090264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACCG1900.01440.6193195
AAGTAGG50.00393136821440.6191195
AAACTGG50.00393136821440.6191195
AACAAGG50.00393136821440.6191195
GAAGCCG800.01440.6191195
TCTACAG50.00393136821440.6191195
TCGCCGT50.00393136821440.6191195
TCTCCCG50.00393136821440.6191195
GTAACCG50.00393136821440.6191195
TCTCCAG7150.01430.5449195
TAATCCG1100.01375.1365195
TAAACCA1500.01344.578195
CCAACCG450.01120.4816195
TATCCAG205.7710655E-5720.3096195
TCCACGG1950.0221.63373195
GTTTGCT27600.0193.876454
CCTGTTT31650.0193.876451
TGTTTGC27500.0193.876453
CCGTTCG17350.0193.876452
TTGATAC3900.0193.876456
Produced by FastQC (version 0.11.5)