FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169850
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA3884822.871945834559906No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2427414.291433617898145No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA133287.846923756255519No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA107446.325581395348837No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA105996.240211951722108No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA103286.080659405357668No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA96875.703267589049161No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA79974.708272004710038No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA71714.221960553429496No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA70134.128937297615543No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA47272.7830438622313807No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA23121.3612010597586104No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA14450.8507506623491315No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA13870.8166028848984397No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA13860.8160141301148071No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA12120.7135707977627318No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA11170.6576390933176331No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA8270.4869002060641742No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA6460.3803355902266706No Hit
TACGGAAGGTTCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA6150.36208419193405944No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4900.28848984397998234No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4720.2778922578745952No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA3450.20312040035325288No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA2710.15955254636443922No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA2670.15719752722990873No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2380.14012363850456286No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCATCTA2150.12658227848101267No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA2080.12246099499558434No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1950.11480718280836033No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCATAAAGGGCATCTA1870.11009714453929938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG159.818821E-6273.17267195
AGATAGT100.0010644551273.17264195
AAGATAG54100.0272.66772195
AAGGCAT3700.0269.48114195
AAGGCAG33150.0265.34418195
AGGCAGG750.0254.96114195
AAGCGGG252.7510578E-7218.53812195
CGTATGT55700.0194.68683
AGGTCCG11550.0194.68687
GTAGGGT1250.0194.68684
TAGGGTG1250.0194.68685
GTCACAA308.367351E-11194.686788
GGGCAAG10800.0194.686789
GGCGCGA10700.0194.686788
GGTCCTG550.0194.686788
GTCGCAA41750.0194.686788
GGCGCAA2000.0194.686788
GGTCCCG3700.0194.686787
TATGTCG55350.0194.686785
TATGTCA308.367351E-11194.686785
Produced by FastQC (version 0.11.5)