FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757
Sequences flagged as poor quality0
Sequence length199-201
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA29939.49801849405548No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA9412.417437252311757No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA506.605019815059446No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA496.472919418758257No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA293.830911492734478No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA172.2457067371202113No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA141.8494055482166447No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA141.8494055482166447No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA131.7173051519154559No Hit
TACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA121.5852047556142668No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA111.453104359313078No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA101.321003963011889No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA101.321003963011889No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA91.1889035667107No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA70.9247027741083224No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA50.6605019815059445No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA50.6605019815059445No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA50.6605019815059445No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA50.6605019815059445No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA50.6605019815059445No Hit
TACGGAGGATGCGAGCGTTATTCGGAATCATTGGGTTTAAAGGGTCTGTA40.5284015852047557No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGCTTAAAGGGAGCGTA40.5284015852047557No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA30.3963011889035667No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA30.3963011889035667No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA30.3963011889035667No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA30.3963011889035667No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA30.3963011889035667No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA20.26420079260237783No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA20.26420079260237783No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA20.26420079260237783No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA20.26420079260237783No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.26420079260237783No Hit
TACAGAGGGTGCAAGCGTTTATCGGAATTACTGGGCGTAAAGCGCGCGTA20.26420079260237783No Hit
GACGAAGGCTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA20.26420079260237783No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA20.26420079260237783No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA20.26420079260237783No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA20.26420079260237783No Hit
TACAGAGGGCGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA10.13210039630118892No Hit
TACAGAGGGGGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA10.13210039630118892No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGAGCGTA10.13210039630118892No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA10.13210039630118892No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10.13210039630118892No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGCGCGTA10.13210039630118892No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.13210039630118892No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA10.13210039630118892No Hit
TACGTCGGTGGCAAGCGTTGTCCGGCATTATTGGGCGTAAAGCGCGCGCA10.13210039630118892No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA10.13210039630118892No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA10.13210039630118892No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA10.13210039630118892No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA10.13210039630118892No Hit
TACAGAGGGTGCAAGCGTTAATCGGCATTACTGGGCGTAACGCGCGCGTA10.13210039630118892No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGAGTGCGCA10.13210039630118892No Hit
GACGAAGGTTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA10.13210039630118892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.13210039630118892No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCTCGTA10.13210039630118892No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGTA10.13210039630118892No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCACGCA10.13210039630118892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGCG10.13210039630118892No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA10.13210039630118892No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGAGCA10.13210039630118892No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA10.13210039630118892No Hit
TACGGGGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA10.13210039630118892No Hit
TACAGAGGGTGCGAGCGTTAATCGGACTTACTGGGCGTAAAGCGAGTGTA10.13210039630118892No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTATTGGGCGTAAAGCGTGCGCA10.13210039630118892No Hit
GACGAAGGATGCAAGCGTTATCCGGCTTCATTGGGTTTAAAGGGAGCGTA10.13210039630118892No Hit
TACGGAGGTGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA10.13210039630118892No Hit
CCATATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAGGCCAC10.13210039630118892No Hit
TACGTAGGGGGCGAGCGTTATCCGAATTTATTGGGCGTAAAGCGTGTGTA10.13210039630118892No Hit
TACGGCGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA10.13210039630118892No Hit
TACGTAGGGTGCGAGCGTTATCCGGATTTACTGGGCGTAAAGGGCGCGTA10.13210039630118892No Hit
TACGTAGGTTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.13210039630118892No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA10.13210039630118892No Hit
TACGGGGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA10.13210039630118892No Hit
TACGTAGTGTGCAAGCGTTAATCGGAATTACTGGGCGTATAGCGTGCGCA10.13210039630118892No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA10.13210039630118892No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTCAAGCGAGCGCA10.13210039630118892No Hit
TACATATGGGGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA10.13210039630118892No Hit
TACGGAGGACGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCACGTA10.13210039630118892No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA10.13210039630118892No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA10.13210039630118892No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.13210039630118892No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.13210039630118892No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGTA10.13210039630118892No Hit
TACGGAAGGTCCGGTCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.13210039630118892No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTGAAGGGCACGCA10.13210039630118892No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTCAAGGGAGCGTC10.13210039630118892No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA10.13210039630118892No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA10.13210039630118892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCAG400.0208.85713195
GGATGCA350.0194.933337
GTGCAAG152.4558734E-5194.933339
TACAGAG152.4558734E-5194.933331
AGGATGC350.0194.933336
GATGCAA350.0194.933338
AGGGTGC252.0227162E-9194.933336
ACGTAGG202.3304347E-7194.933332
CGAAGGA350.0194.933333
CGTAGGG100.0023709128194.933333
TACGTAG202.3304347E-7194.933331
GACGAAG350.0194.933331
AAGGATG350.0194.933335
GTAGGGT100.0023709128194.933334
GTGGCAA100.0023709128194.933338
TAGGGTG100.0023709128194.933335
GTGCGAG100.0023709128194.933339
ACGAAGG350.0194.933332
GGGTGCG100.0023709128194.933337
GGGTGCA152.4558734E-5194.933337
Produced by FastQC (version 0.11.5)