FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169531
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2695115.897387498451609No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2402114.169090018934591No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2223713.116775103078492No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA150828.89630804985519No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA131367.748435389397809No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA119307.0370610684771515No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA81804.825076239743764No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA75654.462310727831488No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA50562.9823454117536023No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA38402.2650724646229894No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA29841.7601500610507814No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA24401.4392647952291913No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA19611.1567205997723131No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA18071.0658817561389953No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA12810.7556140174953253No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA11950.7048858320897063No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA7660.4518347676826067No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA7580.44711586671464215No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA7280.4294199880847751No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA5490.323834578926568No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA5070.2990603488447541No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA4500.2654381794480065No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4010.2365349110192236No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCATAAAGGGCATCTA3370.19878370327550712No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCATAAAGGGCATCTA3260.19229521444455588No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA3090.18226754988763116No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA2640.1557237319428305No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA2380.1403873037969457No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCATAAAGGGCATCTA2130.12564073827205643No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1830.10794485964218935No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA1780.10499554653721148No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1730.10204623343223362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCAGG700.0256.00504195
AGATAGT151.2724024E-5256.00504195
AAGATAG56800.0255.32896195
AAGGCAG48200.0250.69373195
AAGGCAT1000.0243.20477195
AAGCGGG253.8028338E-7204.80402195
AGGTCCA24450.0194.76027
TAGGGCG11150.0194.76025
GAAGGTT8750.0194.76025
GTATGTC58100.0194.760184
ATGGGGC351.8189894E-12194.760186
GTCACAA700.0194.760188
GTCGCGA30750.0194.760188
GGTCCTG600.0194.760188
GTCGCAA26800.0194.760188
TATGTCG57350.0194.760185
TATGTCA700.0194.760185
TACGGAG2000.0194.760181
TACGGAA45800.0194.760181
ACGTAAG153.789484E-5194.760182
Produced by FastQC (version 0.11.5)