FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244020
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA5815723.832882550610606No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2789911.433079255798704No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA192727.897713302188346No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA143705.8888615687238755No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA123165.047127284648799No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA109814.500040980247521No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA106974.383657077288746No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA102914.217277272354725No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA97774.006638800098353No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA74373.047701008114089No Hit
TACGGAAGGTTCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA73102.9956560937628063No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA41861.7154331612162936No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA41751.7109253339890174No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA29001.1884271781001556No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA25991.0650766330628636No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA24891.019998360790099No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA23900.9794279157446111No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA19490.798705024178346No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA15900.6515859355790509No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA13850.5675764281616261No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA12210.5003688222276863No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA8960.36718301778542745No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA8590.35202032620277024No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA8160.33439881976887137No Hit
TACGCATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA8130.33316941234325054No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA7560.3098106712564544No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA6570.26924022621096627No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA5440.22293254651258093No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA5110.20940906483075156No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCG4620.18932874354561102No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA4540.18605032374395541No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA4480.18359150889271372No Hit
TACGCAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA4280.1753954593885747No Hit
TACGGAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA4030.16515039750840096No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3640.1491681009753299No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA3370.13810343414474224No Hit
TACGCAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3110.12744856978936153No Hit
TACGCAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA2970.12171133513646422No Hit
TACATATGGGGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA2920.11966232276042947No Hit
TACGTGTGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2800.11474469305794606No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA2780.11392508810753217No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA2750.11269568068191131No Hit
TACGCATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2730.11187607573149741No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATTTA2670.10941726088025572No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2630.10777805097942793No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA2580.10572903860339317No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA2560.10490943365297928No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTA2550.1044996311777723No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2500.10245061880173756No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCATCTA2450.1004016064257028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGT107.6234667E-4305.4116195
AAGGCAT254.292815E-10305.41156195
AAGGTAG254.292815E-10305.41156195
AAGCCGG254.292815E-10305.41156195
AAGATAG43100.0304.70297195
AAGGCAG68200.0299.14212195
AGGCAGG950.0257.18872195
ACGGCAG251.5788828E-7244.32925195
AAGGCGG29500.0237.0822195
AGGCGAG201.9839232E-5229.0587195
ACTGAAG100.0029381188194.637312
AGTAGGT100.0029381188194.637313
GTCGCAA31900.0194.637318
AGGGCCC100.0029381188194.637316
AGGTGCC100.0029381188194.637316
CTGAAGG100.0029381188194.637313
GGCCCGA100.0029381188194.637318
TACTGAA100.0029381188194.637311
TCGCAAG31900.0194.637319
AGGTTGC204.910089E-7194.637316
Produced by FastQC (version 0.11.5)