FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365
Sequences flagged as poor quality0
Sequence length194-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA9325.47945205479452No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA7019.17808219178082No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA246.575342465753424No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA123.287671232876712No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA113.0136986301369864No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA82.191780821917808No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA71.9178082191780823No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA71.9178082191780823No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA71.9178082191780823No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA61.643835616438356No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA51.36986301369863No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA51.36986301369863No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA51.36986301369863No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA41.095890410958904No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA41.095890410958904No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA41.095890410958904No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA30.821917808219178No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA30.821917808219178No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA30.821917808219178No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA30.821917808219178No Hit
GACGTAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA30.821917808219178No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.547945205479452No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA20.547945205479452No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA20.547945205479452No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA20.547945205479452No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA20.547945205479452No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA20.547945205479452No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA20.547945205479452No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.547945205479452No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA20.547945205479452No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA20.547945205479452No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA20.547945205479452No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA20.547945205479452No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA20.547945205479452No Hit
TACGTAGGTTGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA10.273972602739726No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA10.273972602739726No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.273972602739726No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA10.273972602739726No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA10.273972602739726No Hit
TACAGAGGGTGCGAGCGTTATTCGGATTTACTGGGCGTAAAGCGTGCGTA10.273972602739726No Hit
TACGTAGGTGGCAAGCGTTGTCCGGGATTATTGGGCGTAAAGCGCGCGCA10.273972602739726No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA10.273972602739726No Hit
CTAACAATTCAGCGGCTTTAACCGGACCCTCGACGCCATTAATATTGTTT10.273972602739726No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA10.273972602739726No Hit
TACGTAGGATGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.273972602739726No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGCGTGCGCA10.273972602739726No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCGCGCA10.273972602739726No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA10.273972602739726No Hit
TACGTAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTGAAGCGTGCGCA10.273972602739726No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA10.273972602739726No Hit
TACGTAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA10.273972602739726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.273972602739726No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA10.273972602739726No Hit
GCCAGCAATCTCTTTTTGAGTCTCATTTTGCATCTCGGCAATCTCTTTCT10.273972602739726No Hit
TACGTAGGTCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA10.273972602739726No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA10.273972602739726No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTA10.273972602739726No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGAGCTCGTA10.273972602739726No Hit
TACGTAGGTTGCAAGCGTTAATCGGAACTACTGGGCGTAAAGCGTGCGCA10.273972602739726No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA10.273972602739726No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA10.273972602739726No Hit
TACGCAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.273972602739726No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGGGAGCGCA10.273972602739726No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGAGCGCA10.273972602739726No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGCGTA10.273972602739726No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.273972602739726No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA10.273972602739726No Hit
ATCTTGATTACGCTCATTAGGGTTAGCCTCGTTACGGTCAGGCATCCACG10.273972602739726No Hit
TACGGAAGGTCCTGGTGTTATCCGGATCTATTGGGTTTAAAGGGAGCGTA10.273972602739726No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA10.273972602739726No Hit
TACATATGGGGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA10.273972602739726No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATAATTGGGCGTAAAGGGCGCGTA10.273972602739726No Hit
TACGGAGGATTCAAGCATTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.273972602739726No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA10.273972602739726No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA10.273972602739726No Hit
TACGGAAGTCCCGAGCGTTGTCCGGATTTATTGGGCTTAAAGCGAGCGCA10.273972602739726No Hit
TACGGAAGGTCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.273972602739726No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGCTTAAAGGGAGCGTA10.273972602739726No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTG10.273972602739726No Hit
TACGGAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA10.273972602739726No Hit
TACGGAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.273972602739726No Hit
ACCCCTGCCTACAGTATTGTTATCGTTAGCAAGCACATCACCTTGTATTC10.273972602739726No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCTTAAAGCGAGCGCA10.273972602739726No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA10.273972602739726No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.273972602739726No Hit
TACGCAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.273972602739726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGG100.0010295877227.33333195
AAGGCAG202.1267624E-8227.33333195
GGATGCA151.33481535E-5194.857157
AGGATGC151.33481535E-5194.857156
GATGCAA151.33481535E-5194.857158
ACGTAGG207.425842E-8194.857152
CGAAGGA100.0017997974194.857153
CGTAGGG100.0017997974194.857153
TACGTAG151.33481535E-5194.857151
GACGAAG100.0017997974194.857151
ATGCAAG151.33481535E-5194.857159
AAGGATG100.0017997974194.857155
ACGAAGG100.0017997974194.857152
GAAGGAT100.0017997974194.857154
CCGGATT200.00107199638.97142820-24
CGTTATC255.8900252E-538.97142815-19
GCAAGCG303.1519812E-638.97142810-14
TTGTAAG200.00107199638.97142855-59
TGCAAGC200.00107199638.97142810-14
CACCGAT200.00107199638.971428180-184
Produced by FastQC (version 0.11.5)