FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88879
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG937410.546923345222156No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG83039.341914287964537No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA82689.302534907008404No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA75538.49807041033315No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA47465.339844057651414No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC45445.112568773276028No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG38824.367735910620056No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG36124.063952114672757No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG34693.9030592153377066No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA32063.6071512955816334No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG27283.0693414642378967No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG14341.613429494031211No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG13791.551547609671576No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC10151.1420020477278097No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA10131.1397517973874594No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA9191.0339900313909922No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG9101.0238639048594156No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG7440.8370931266103354No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA7050.7932132449735033No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA6660.7493333633366712No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA5990.673949976934934No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA5360.6030670912138976No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA5250.5906907143419705No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA4910.5524364585560143No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC4300.4838038231753283No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4060.4568008190911239No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG4060.4568008190911239No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG3960.44554956738937207No Hit
CCTGTTCGATACCCACGCTTTCGAGCATCAGCGTCAGTTGCGCTACAGTA3720.4185465633051677No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG3410.3836676830297371No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA3350.376916932008686No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA3320.3735415564981604No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA3220.3622903047964086No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAACGTCAGTGTTATCCCAGGA3120.35103905309465677No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGAG2920.32853654969115315No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA2800.31503504764905094No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA2760.31053454696835026No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2610.2936576694157225No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC2300.2587787891402919No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA1980.22277478369468603No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGTA1860.20927328165258385No Hit
CCTGTTCGATACCCACGCTTTCGAGCATCAGCGTCAGTTGTGCTACAGTA1830.20589790614205827No Hit
CCTGTTTGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC1710.19239640409995612No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC1640.18452052790872986No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1630.18339540273855465No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA1610.1811451523982043No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAGCGTCAGTGTTATCCCAGGA1590.17889490205785394No Hit
CCTGTTTGCTACCCACGCTTTCGTGCTTTAGCGTCAGTATCTGTCCAGTA1570.17664465171750357No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1450.1631431496754014No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA1430.16089289933505102No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTTATCCCAGGA1420.15976777416487586No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA1400.15751752382452547No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1370.15414214831399994No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTAAAAGTCCAGAA1310.14739139729294884No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAAGCCAGAG1300.14626627212277366No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA1290.14514114695259847No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG1270.1428908966122481No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTGAACGTCAGTGTTATCCCAGGA1270.1428908966122481No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG1210.13614014559119703No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGGCCAAAA1200.13501502042102184No Hit
CCTGTTTGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTACCGTCCAGTG1180.13276477008067147No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAATCCCGGCCTGGCC1160.1305145197403211No Hit
CCTGTTTGCTCCCCACGCTTTCGTACATGAGCGTCAGTACATTCCCAAGG1110.12488889388944521No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTTCTGTCCAGTG1100.12376376871927003No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTATAGTCCAGAA1000.11251251701751819No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATTGGCCAGTA970.10913714150699266No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG970.10913714150699266No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG930.10463664082629193No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG900.10126126531576637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCCG350.0344.7059195
GAGGCGG600.0344.70587195
GGACCCG105.2945444E-4344.70587195
ATAACCG400.0344.70587195
ATTGCCG550.0344.70587195
AAAACCG105.2945444E-4344.70587195
GTAACCG8100.0344.70587195
GGAACCG1250.0344.70587195
ATATCCG1600.0344.70587195
ACAACCG202.816887E-8344.70587195
CTGGCCG3550.0339.85086195
CCAACCG7800.0333.6576195
GTCACCG3400.0329.4983195
GCAACCG1100.0329.0374195
CCAGCCG1200.0315.9804195
CAAACGG400.0301.61765195
TAAACCA2850.0290.27863195
TAATCCG900.0287.2549195
TCGTCCG306.148184E-10287.2549195
GAAGCCG258.575989E-8275.7647195
Produced by FastQC (version 0.11.5)