FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176308
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA2652315.043560133402908No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2102111.922884951335163No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1826310.358577035642172No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC144668.204959502688478No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG114046.468226058942306No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG85124.827914785489031No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG66273.7587630737119135No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC60633.4388683440343035No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG45912.603965787145223No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA43202.4502575039136056No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG38152.1638269392200016No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG37442.1235565033917916No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA33271.8870385915556867No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG24931.4140027678834766No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA24771.4049277400912041No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA23521.3340290854640744No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA21011.1916645869727975No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA17791.0090296526533113No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA15610.8853823989835968No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC13970.7923633641128027No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA12780.724867844907775No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC12080.6851645983165823No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA9960.5649204800689702No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG9240.5240828550037434No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA7490.42482473852576175No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA7170.4066746829412165No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA6220.35279170542459787No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG5870.33294008212900156No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA5650.3204619189146267No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATTGTCTGTTAGTC4870.27622115842729766No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC4770.2705492660571273No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTT4610.26147423826485466No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA4380.24842888581346279No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4280.24275699344329243No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA3810.21609909930349164No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA3800.21553191006647457No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA3380.19170996211175897No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA3250.18433650203053745No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA3070.17412709576423077No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA2820.1599473648388048No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGCTATCCCAGGG2700.15314109399460035No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC2500.1417973092542596No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA2460.13952855230619143No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT2460.13952855230619143No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT2340.13272228146198697No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2230.12648319985479956No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTTATCCCAGGA2120.12024411824761214No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG2120.12024411824761214No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA2110.11967692901059508No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA1900.10776595503323728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG6650.0290.8479195
TCTGCGG550.0290.8479195
TCGTCCG1250.0290.8479195
GGAACCG1100.0290.8479195
CAAACGG450.0290.8479195
GTCACCG15950.0290.84787195
CCAGCCG2400.0290.84787195
AGGGCCG157.6444885E-6290.84787195
GCAACCG650.0290.84787195
CTGGCCG5450.0290.84787195
TAACCGT108.8221027E-4290.84787195
ATAGCCG350.0290.84787195
ATTGCCG255.7480065E-10290.84787195
CGAACCG108.8221027E-4290.84787195
TAATCCG1600.0290.84787195
ATTTCCG206.613118E-8290.84787195
AAGTCCG800.0290.84787195
ATATCCG12400.0289.6751195
GTAACCG27350.0288.721195
TAGCCCG700.0270.07303195
Produced by FastQC (version 0.11.5)