FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125425
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA3036924.212876220849115No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2793822.27466613514052No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA2493619.88120390671716No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA1600112.757424755830177No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA102188.146701215866054No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA36722.9276460035878014No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA27432.1869643213075545No Hit
TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA10530.8395455451465019No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA7880.6282639027307155No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGTGCA7790.6210882997807454No Hit
GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTA4900.3906717161650389No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGAGTGCA2260.1801873629659159No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1860.1482957942993821No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1830.14590392664939209No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGCGCA1430.11401235798285828No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGCTTGTA1430.11401235798285828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGCA100.0010879887271.14658195
AAGGCGG72750.0267.23312195
AAGCGGG950.0242.60483195
GAAGGCA14000.0230.4746195
GGCGCGA12150.0194.710948
GGTCCCG32700.0194.710947
GCGCGAG12150.0194.710949
CGTAGGG12950.0194.710943
AGGTCCC32700.0194.710946
TCCCGAG32700.0194.710949
GTATGTC100.0029331024194.710924
AGGGGGC100.0029331024194.710926
GTATGGC100.0029331024194.710924
GGATGCA1050.0194.710927
AACGTAG204.891426E-7194.710921
GGTTGCA153.7909653E-5194.710927
TACAGAG1200.0194.710921
AGGATGC1050.0194.710926
GTCGCGA308.367351E-11194.710928
GTCGCAA204.891426E-7194.710928
Produced by FastQC (version 0.11.5)