FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125425
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT2781622.177396850707595No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2672921.310743472194538No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC2298518.325692645006978No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG1781114.200518237990831No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG106188.465616902531394No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG36732.928443292804465No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA10260.8180187362965916No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG9800.7813434323300777No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAC5860.46721148096472No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG5280.4209687063982459No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3790.3021726131154076No Hit
CCCGTTCGCTCCCCATGCTTTCGCACTCCAGCGTCGGTAGGGACCCAGAG3720.2965915885987642No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3560.2838349611321507No Hit
CCTGTTCGCTACCCATGCTTTCGCGTCTCAGCGTCAGTTGCAGACCAGGT3420.27267291209886385No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGGT2800.2232409806657365No Hit
CCTGTTCGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAC2580.2057006178991429No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGAG2470.1969304365158461No Hit
CCCGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAC2160.17221447079928245No Hit
CCTGTTCGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTGCAGACCAGAG2120.16902531393262907No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGG1620.12916085309946185No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1350.10763404424955153No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTACAGACCAGAG1320.10524217659956148No Hit
CCTGTTCGCTACCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1310.10444488738289816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG153.6839901E-6349.04584195
ATAACCG105.104054E-4349.04584195
AATACCG105.104054E-4349.04584195
AAAGCCA1050.0349.04584195
ACAACCG202.6539055E-8349.04584195
AAAACCG49000.0348.6897195
TGAACCG13900.0347.79028195
CGAACCG650.0322.19617195
GCAACCG750.0302.50638195
TAAACCG201.16166575E-5261.7844195
TTAACCG201.16166575E-5261.7844195
CATTCCG3500.0254.30484195
AACACCG150.0017189303232.69724195
CGTTCGC800.0194.285493
ACTGTTC100.0029523629194.285491
GTTTGCT4350.0194.285494
CGATACC204.945032E-7194.285497
TCGATAC204.945032E-7194.285496
CCTGTTC118000.0194.285491
TTCGATA204.945032E-7194.285495
Produced by FastQC (version 0.11.5)