FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309
Sequences flagged as poor quality0
Sequence length200-201
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA10132.68608414239482No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA206.472491909385113No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA165.177993527508091No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA154.854368932038835No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA123.8834951456310676No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA123.8834951456310676No Hit
GACGTAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA92.912621359223301No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA82.5889967637540456No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA72.26537216828479No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA61.9417475728155338No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA61.9417475728155338No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA51.6181229773462782No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA41.2944983818770228No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA41.2944983818770228No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA41.2944983818770228No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA41.2944983818770228No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA30.9708737864077669No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA30.9708737864077669No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA30.9708737864077669No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA30.9708737864077669No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA20.6472491909385114No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA20.6472491909385114No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA20.6472491909385114No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA20.6472491909385114No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA20.6472491909385114No Hit
TACGGAGGGTCCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA20.6472491909385114No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.6472491909385114No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA20.6472491909385114No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA20.6472491909385114No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA20.6472491909385114No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA20.6472491909385114No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA20.6472491909385114No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA20.6472491909385114No Hit
TACGTAGGTCCCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA20.6472491909385114No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA10.3236245954692557No Hit
TACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA10.3236245954692557No Hit
TACGTAGGTCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTCGCA10.3236245954692557No Hit
TACAGAGGGTGCGAGCGTTATTCGGATTTACTGGGCGTAAAGCGTGCGTA10.3236245954692557No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA10.3236245954692557No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGTGTA10.3236245954692557No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA10.3236245954692557No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.3236245954692557No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA10.3236245954692557No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGTA10.3236245954692557No Hit
TACGTATGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA10.3236245954692557No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGTGCA10.3236245954692557No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA10.3236245954692557No Hit
TACGGAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA10.3236245954692557No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA10.3236245954692557No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGCA10.3236245954692557No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA10.3236245954692557No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.3236245954692557No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA10.3236245954692557No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA10.3236245954692557No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA10.3236245954692557No Hit
TACGGAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA10.3236245954692557No Hit
GACGAAGGCTGCAAGCGTTATCCGGATTCATTGGGTTTTAAGGGAGCGTA10.3236245954692557No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10.3236245954692557No Hit
TACGGCGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA10.3236245954692557No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA10.3236245954692557No Hit
TACGTAGGGTGCGAGCGTTATTCGGATTTACTGGGCGTAAAGCGAGCGCA10.3236245954692557No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCATAAAGGGCATCTA10.3236245954692557No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGCA10.3236245954692557No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGCA10.3236245954692557No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCGCGCA10.3236245954692557No Hit
TACGTAGGTGGCAAGCATTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA10.3236245954692557No Hit
TACGTATGTCGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGCGTGTA10.3236245954692557No Hit
TACGGAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.3236245954692557No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGTA10.3236245954692557No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGCGCGTA10.3236245954692557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGCA204.5782144E-8195.07
AGGATGC204.5782144E-8195.06
GATGCAA204.5782144E-8195.08
CGAAGGA204.5782144E-8195.03
AAGGCGG100.0016304634195.0195
AAGGCAG204.5782144E-8195.0195
GACGAAG204.5782144E-8195.01
ATGCAAG204.5782144E-8195.09
AAGGATG204.5782144E-8195.05
ACGAAGG204.5782144E-8195.02
GAAGGAT204.5782144E-8195.04
AGGGAGC300.007441332664.999996
TGAGAAA200.001015494239.090-94
TAGAACA200.001015494239.0175-179
TTCATTG200.001015494239.025-29
GAGCGTA200.001015494239.040-44
ACTGCCA200.001015494239.095-99
ACTGCAG200.001015494239.0105-109
GGGTTTA200.001015494239.030-34
AAACTGC200.001015494239.095-99
Produced by FastQC (version 0.11.5)