FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276
Sequences flagged as poor quality0
Sequence length200-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA6222.463768115942027No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4114.855072463768115No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA3111.231884057971014No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA155.434782608695652No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA145.072463768115942No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA103.6231884057971016No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA93.260869565217391No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA72.536231884057971No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA62.1739130434782608No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA62.1739130434782608No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA41.4492753623188406No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA41.4492753623188406No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA31.0869565217391304No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA31.0869565217391304No Hit
GACGTAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA31.0869565217391304No Hit
TACAGAGGGCGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA20.7246376811594203No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA20.7246376811594203No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGTGCGTAAAGCGAGCGCA20.7246376811594203No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA20.7246376811594203No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.7246376811594203No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA20.7246376811594203No Hit
TACAGAGGGTGCGAGCGTTACTCGGAATTACTGGGCGTAAAGCGAGTGTA20.7246376811594203No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA20.7246376811594203No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA10.36231884057971014No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.36231884057971014No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGCAAAGCGTGCGCA10.36231884057971014No Hit
TACGTAGAGCGCAAGCGTTGTCCGGAATTCTTGGGCGTAAAGAGCTCGTA10.36231884057971014No Hit
TACGTAGGTGCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.36231884057971014No Hit
TACAGAGGGTGCGAGCGTTATTCGGATTTACTGGGCGTAAAGCGTGCGTA10.36231884057971014No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA10.36231884057971014No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA10.36231884057971014No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACCGGGCGTAAAGGGTGCGTA10.36231884057971014No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA10.36231884057971014No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA10.36231884057971014No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA10.36231884057971014No Hit
TACGTAGGTCGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA10.36231884057971014No Hit
TACGTAGCGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.36231884057971014No Hit
TACGTAGGGCGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA10.36231884057971014No Hit
TACGTAGGTCGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA10.36231884057971014No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA10.36231884057971014No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA10.36231884057971014No Hit
TACGTAGGTCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTCGTA10.36231884057971014No Hit
TACGTAGGTGGCAAGAGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA10.36231884057971014No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGCA10.36231884057971014No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA10.36231884057971014No Hit
TACGTAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA10.36231884057971014No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA10.36231884057971014No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGCGCGCA10.36231884057971014No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA10.36231884057971014No Hit
TACGGACGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.36231884057971014No Hit
TACGGAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.36231884057971014No Hit
TACAGGGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA10.36231884057971014No Hit
TACGTAGGTTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA10.36231884057971014No Hit
TACGGGGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA10.36231884057971014No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA10.36231884057971014No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA10.36231884057971014No Hit
TACGTAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA10.36231884057971014No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGTGTA10.36231884057971014No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGCGCGCGCA10.36231884057971014No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA10.36231884057971014No Hit
TACGGAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.36231884057971014No Hit
TACGTAGGGCGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.36231884057971014No Hit
TACGGAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.36231884057971014No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGCA10.36231884057971014No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTATTGGGCGTAAAGGGCTCGTA10.36231884057971014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGGCA100.0014098041195.00002195
ACAGAGG157.3246374E-6195.02
TACAGAG157.3246374E-6195.01
AGAGGGT157.3246374E-6195.04
CAGAGGG157.3246374E-6195.03
AAGGCGG157.3246374E-6195.0195
GTGCGAG157.3246374E-6195.09
GAGGGTG157.3246374E-6195.05
GGTGCGA157.3246374E-6195.08
AGGGTGC202.3130428E-5146.250026
GGGTGCG202.3130428E-5146.250027
TGTGAAA209.447528E-439.00000470-74
GATACTG209.447528E-439.000004105-109
GAAATCC209.447528E-439.00000470-74
CTGGGCG209.447528E-439.00000430-34
GTGTAGC209.447528E-439.000004150-154
TAAGTCA209.447528E-439.00000460-64
TGATACT209.447528E-439.000004105-109
ATGTGAA209.447528E-439.00000465-69
TGTAGCG209.447528E-439.000004150-154
Produced by FastQC (version 0.11.5)