FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81734
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1358616.622213521912546No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA71818.785817407688356No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC61697.5476545868304505No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG51746.330290943793281No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA51196.26299948613796No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA46125.642694594660728No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC44875.489759463625908No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG42665.219370151956347No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG32814.014241319401962No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA23392.861722171923557No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA18372.247534685687719No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG17272.1129517703770766No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG17112.09337607360462No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA15741.925759169990457No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA11841.4486015611618175No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC11661.4265789022928035No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA10931.3372647857684685No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCATCCAGTG9921.2136931998923337No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG7890.9653265470917857No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA7760.9494212934641642No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA4530.5542369148701887No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG3920.4796045709251964No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC3760.46002887415273935No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA3720.4551349499596251No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA2620.3205520346489833No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA2410.2948589326351335No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG2390.29241197053857637No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2370.2899650084420192No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGCTGTCCAGTA1860.22756747497981256No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1790.21900310764186265No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA1780.21777962659358407No Hit
CCTGTTCGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG1750.21410918344874838No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1520.18596911933834145No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1400.1712873467589987No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1140.13947683950375608No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAACGTCAGTTATCGTCCAGTA1030.1260185479726919No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC960.11745418063474197No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG850.1039958891036778No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG850.1039958891036778No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC820.1003254459588421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGG103.3959324E-4399.67886195
CTGACCG201.3424142E-8399.67886195
TCTGCGG103.3959324E-4399.67886195
TCGCCGG103.3959324E-4399.67886195
TATCCAG103.3959324E-4399.67886195
GTAACCG201.3424142E-8399.67886195
GGAACCG103.3959324E-4399.67886195
GTCACCG300.0399.67883195
CCAGCCG600.0399.67883195
CTGGCCG8050.0399.67883195
CGAACCG1250.0399.67883195
TAATCCG300.0399.67883195
TCGTCCG1300.0399.67883195
GAAGCCG750.0399.6788195
TCTCCGG7600.0386.5315195
CAAGCCG5900.0382.7433195
TCCACGG2150.0381.0891195
CCAACCG1250.0367.70453195
ATATCCG2050.0360.6858195
TTCGCCG1000.0279.7752195
Produced by FastQC (version 0.11.5)