FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43573
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1634937.52094186767035No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA671415.40862460698139No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA36568.390517063319027No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA29466.761067633626327No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA27096.2171528239965115No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA24255.565373052119432No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA12472.8618639983476006No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA11892.728754044936084No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10292.3615541734560392No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA7511.7235443967594613No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA7101.6294494296926996No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA4260.9776696578156198No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA3020.6930897574185849No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA2760.6334197783030776No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA1990.4567048401533059No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGTGTAAAGCGCGCGCA1780.40850985702155No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA1120.2570399100360315No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA830.19048493333027333No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA740.1698299405595208No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA640.146879948592018No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA590.1354049526082666No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA490.11245496064076377No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA480.11015996144401351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG259.094947E-12579.6747195
AAGCGGG202.05182E-9579.6747195
AAGGCAG9850.0576.7321195
AAGGCGG1800.0466.9601195
GTATGTC351.8189894E-12194.33414
GTCACGA100.0029435712194.33418
GGTCCTG100.0029435712194.33417
GTCGCAA351.8189894E-12194.33418
GGTCCGG3000.0194.33418
GTCCGGG3000.0194.33419
TATGTCG351.8189894E-12194.33415
TACGGAG1550.0194.33411
AGGGTGC3550.0194.33416
GGTTCGG700.0194.33418
GGTCACG100.0029435712194.33417
GTCCTGA100.0029435712194.33418
TACGTAT400.0194.33411
TCACGAG100.0029435712194.33419
CGTATGT351.8189894E-12194.33413
AAGGTTC700.0194.33416
Produced by FastQC (version 0.11.5)