FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111384
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1498913.457049486461251No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC1328211.92451339510163No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG89668.049630108453638No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA86477.763233498527616No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG61275.500790059613589No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG54884.927099044746104No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA54274.8723335488041375No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC51424.616461969403146No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC40823.6647992530345475No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG32222.892695539754363No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG32132.8846153846153846No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG31992.8720462543991956No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA27692.4859943977591037No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG20811.8683114271349566No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA17871.6043596925949866No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG13431.205738705738706No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA12631.1339151045033398No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA11261.0109171873877756No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA11110.9974502621561444No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA11050.992063492063492No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA9000.808015513897867No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC8620.773899303311068No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA7150.641923436041083No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA6030.5413703943115707No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC5200.4668534080298786No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA4950.44440853264382674No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4750.42645263233498526No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA3990.35822021116138764No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA3680.3303885656826833No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA3670.32949077066724125No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA3470.3115348703583998No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA2840.2549737843855491No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA2680.2406090641384759No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA2470.22175536881419233No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA1950.1750700280112045No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1930.17327443798032033No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1740.15621633268692092No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATTGTCTGTTAGTC1690.15172735760971057No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTAAAAGTCCAGAA1190.10683760683760685No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA1170.10504201680672269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG1600.0346.0767195
CCAGCCG400.0346.0767195
GCAACCG1100.0346.0767195
ATAGCCG105.235073E-4346.0767195
CGAGCCG105.235073E-4346.0767195
GTAACCG9700.0346.0767195
ATTTCCG105.235073E-4346.0767195
AAGTCCG252.0190782E-10346.0767195
GTCACCG4350.0346.07666195
CTGGCCG5750.0346.07666195
ATTGCCG301.8189894E-12346.07666195
TAATCCG1400.0346.07666195
TCGTCCG750.0346.07666195
GGAACCG1300.0346.07666195
CAAACGG301.8189894E-12346.07666195
TCTCCGG6450.0319.24902195
GAAGCCG950.0273.21844195
TTCGCCG1250.0249.1752195
CAAGCCG2300.0240.749195
TAGCCCG150.0017630229230.71777195
Produced by FastQC (version 0.11.5)