FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648
Sequences flagged as poor quality0
Sequence length199-201
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG43416.389728096676738No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG31611.933534743202417No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT28310.687311178247734No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG2087.854984894259818No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG963.625377643504532No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGTGTCAGATGTCGCCTAGGC853.2099697885196377No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG722.719033232628399No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA351.321752265861027No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTACCAGCCTAGTC301.1329305135951662No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAACCGGACCAGAC291.095166163141994No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCT281.0574018126888218No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGCTGGTCCAGGT271.0196374622356497No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG271.0196374622356497No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAACGGACCAGTA260.9818731117824773No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT250.9441087613293051No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG250.9441087613293051No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGCGGGCCCAGCG240.906344410876133No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGTC220.8308157099697886No Hit
CCTGTTTGCTCCCCACACTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG220.8308157099697886No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGAGCCAGGT210.7930513595166163No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA200.7552870090634441No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGAA200.7552870090634441No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG190.7175226586102719No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG180.6797583081570997No Hit
CCCGTTTGCTCCCCTGGCTTTCGTTCCTCAGTGTCAGAAACAGTCCAGAA180.6797583081570997No Hit
CCCGTTTGCTACCCTGGCTTTCGTGCCTCAGCGTCAGGAACTGTCTAGAG180.6797583081570997No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGTGTCAGTATTAGGCCAGAT170.6419939577039275No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACATGTCCAGTC150.5664652567975831No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA150.5664652567975831No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA150.5664652567975831No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG140.5287009063444109No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCACAAATCGTCCAGGT140.5287009063444109No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTCGTCCCAGGG140.5287009063444109No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG130.49093655589123864No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTAGTGGCCCAGAG120.4531722054380665No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGAGCCAGTA120.4531722054380665No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCTAGCA120.4531722054380665No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAACAGTCGTCCAGTG120.4531722054380665No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGTAATGGACCAGGT120.4531722054380665No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG120.4531722054380665No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCATCAGCGTCAATACCGGTCCAGGA120.4531722054380665No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTACCGGCCCAGAG110.4154078549848943No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG100.3776435045317221No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG100.3776435045317221No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTACAGGCCCAGGG90.33987915407854985No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG90.33987915407854985No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG90.33987915407854985No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGTGTCAGTATCTGTCCAGGG80.3021148036253776No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATCTTGACCCAGGG80.3021148036253776No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG80.3021148036253776No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTAAATCTTAGTG70.26435045317220546No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTGCAGGCCCAGGG60.22658610271903326No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA60.22658610271903326No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA60.22658610271903326No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTC60.22658610271903326No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACAGGCCCAGGG50.18882175226586104No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT50.18882175226586104No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTCCGGACCAGTA50.18882175226586104No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACCGGCCCAGGG50.18882175226586104No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTGTTATCCCAGGG50.18882175226586104No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG50.18882175226586104No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTCAGGCCCAGGC50.18882175226586104No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAA50.18882175226586104No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC40.1510574018126888No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGTG40.1510574018126888No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC40.1510574018126888No Hit
CCTTATTGCTACCCAAGCCTTCGTCCCTCAACGTCAGTTTTTACATAGAA40.1510574018126888No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCTTGGTCCAGGA40.1510574018126888No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTGAGTGTCAGTGACGGCCTAGCA40.1510574018126888No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTA40.1510574018126888No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA40.1510574018126888No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTATCCCAGGG30.11329305135951663No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG30.11329305135951663No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG30.11329305135951663No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTATCCCAGGG30.11329305135951663No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA30.11329305135951663No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAGCGTCAGTGCAGGCCCAGGG30.11329305135951663No Hit
CCTGTTCGCTCCCCACACTTTCGCTCCTCAGCGTCAGGATATTCCCAGAG30.11329305135951663No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATGAGCCAGTA30.11329305135951663No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGA30.11329305135951663No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA30.11329305135951663No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA30.11329305135951663No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA30.11329305135951663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG100.0015464345235.60466195
GTCACCG100.0015464345235.60466195
AAAACCG700.0235.60466195
AAAGCCG100.0015464345235.60466195
ACAACCG251.4206307E-9235.60463195
ATCCCCA153.37199E-5194.826929
GTTTGCT1950.0194.826924
GTTTGAT153.37199E-5194.826924
CCTGTTT1850.0194.826921
CCTGTTC400.0194.826921
CTCCCCA1950.0194.826929
TGATCCC153.37199E-5194.826927
TGTTCGC350.0194.826923
TGTTTGC1700.0194.826923
TGTTTGA153.37199E-5194.826923
GGTTTGC204.0229315E-7194.826923
CCCATTC100.0027656355194.826921
CATTCGC100.0027656355194.826923
GATCCCC153.37199E-5194.826928
CCATTCG100.0027656355194.826922
Produced by FastQC (version 0.11.5)