FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118946
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1940416.31328502009315No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1588313.353118221714055No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1558613.103425083651404No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA1264010.626670926302692No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG95328.013720511828897No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC88507.440351083685033No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG60195.060279454542398No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG47934.029559632101963No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG25422.137104232172583No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG23741.995863669228053No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA18321.5401947102046307No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC8050.676777697442537No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC6880.5784137339633111No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA5190.43633245338220705No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4850.4077480537386713No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA4620.388411548097456No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA4310.362349301363644No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG3960.3329241840835337No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA3550.29845476098397594No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA3520.2959326080742522No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG3340.28079969061590976No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA2870.24128596169690447No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA2780.23371950296773325No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA2630.22110873841911455No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA2530.21270156205336876No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2170.18243572713668388No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA2140.17991357422696014No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA1900.1597363509491702No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG1770.1488070216737007No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA1760.1479663040371261No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA1680.14124056294452944No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG1620.13619625712508196No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1470.12358549257646327No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTGAACGTCAGTGTTATCCCAGGA1350.11349688093756831No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCACGG1320.11097472802784458No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1220.10256755166209877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCG16900.0455.51428195
CCAGCCG151.2700966E-6455.51428195
GCAACCG350.0455.51428195
ATTGCCG151.2700966E-6455.51428195
GGAACCG1750.0455.51428195
AATCCGG102.2969011E-4455.51425195
GAAGCCG102.2969011E-4455.51425195
GACACCG102.2969011E-4455.51425195
CTGGCCG400.0455.51425195
CAAACGG207.008566E-9455.51425195
GTAACCG13050.0453.769195
TAAACCA2500.0428.1834195
CCAACCG800.0427.04462195
TCTCCGG1650.0414.1039195
TCTCCAG10450.0411.9244195
TAATCCG600.0379.59525195
AAAGCCA401.8189894E-12341.63568195
TCTCCCG157.7393284E-4303.67618195
TTGATAC9900.0194.426856
ATACCCG11950.0194.426859
Produced by FastQC (version 0.11.5)