FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG17330.565371024734983No Hit
CCTGTTTGCTACCCACGCTTTCGAATCTCAGTGTCAATATTATGCCAGGA366.36042402826855No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGGCCAGTG284.946996466431095No Hit
CCTGTTTGATCCCCGCACTCTCGTGCCTGAGCGTCAATACTGGCCCAGCC223.8869257950530036No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG183.180212014134275No Hit
CCTGTTCGCTACCCCAGCCTTCGCGCCTCAGCGTCAGTTACAGGCCAGAA173.0035335689045937No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTATCAGCCCAGCA173.0035335689045937No Hit
CCTGTTTGATCCCCGCACTTTCGAGCCTCAGTGTCAGTTACAGTTTTGTG162.8268551236749118No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG152.65017667844523No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG122.1201413427561837No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT111.9434628975265018No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA111.9434628975265018No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGAGCCAGGT101.76678445229682No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC91.5901060070671376No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG91.5901060070671376No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG91.5901060070671376No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG81.4134275618374559No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGAAACGGACCAGCT61.0600706713780919No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTGTTATCCCAGGG40.7067137809187279No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG30.5300353356890459No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGTGTCAATGACCGTGTAGTG30.5300353356890459No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTATCCCAGGG30.5300353356890459No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG20.35335689045936397No Hit
CCTGTTCGATGCACAAAGCCTTCGTCCTTCAACGTCAGTTATCACATAAG20.35335689045936397No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT20.35335689045936397No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC20.35335689045936397No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG20.35335689045936397No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT20.35335689045936397No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTCCGTCCAGAA20.35335689045936397No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACCGGCCCAGAG20.35335689045936397No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG20.35335689045936397No Hit
CCTGTTCGATCCCCACACTTTCGAGCTTCAGCGTCAGTTACACTCCAGTG10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGAATCGGACCAGCT10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGAGTTATCTCAGGG10.17667844522968199No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGTGTCAGTTCAGGCCCAGGC10.17667844522968199No Hit
CCTGTTCGCTCCCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCTTTCGCCCCTCAGCGTCAGTTACATCCCAGAG10.17667844522968199No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA10.17667844522968199No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG10.17667844522968199No Hit
ACTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTGTTATCCCAGGG10.17667844522968199No Hit
CCCGTTTGCTACCCTCGCTTTCGCGCCTCAGCGTCAGTATCAGCCCAGCA10.17667844522968199No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACGCCCCAGCA10.17667844522968199No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTC10.17667844522968199No Hit
CGACTGCCTATGATGTTTATCCTTTGGATGGTCGCCATGATGGTGGTTAT10.17667844522968199No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTTATGGCTTAGAG10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTTAGCGTCAGTTGCGTTCCAGCA10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCCTTCGAGCCTCAGCGTCAGTTACAGTCCCGTG10.17667844522968199No Hit
CCTGTTCGCTACCCTCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCTTC10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCTTTCGATTCTCAGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA10.17667844522968199No Hit
CCTGTTCGCTACCCATGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAC10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTAGTGGACCAGTA10.17667844522968199No Hit
CCTGTTTGCTACACACGCTTTCGAATCTCAGTGTCAATATTATGCCAGGA10.17667844522968199No Hit
CCTGTTCGCTACCCACACCTTCGAGCTTCAGCGTCAGTTACGCTCCAGTC10.17667844522968199No Hit
CCTGTTTGCTCCCCGCACCTTCGAACCTCAGCTTCAATTTTGCTCCATTC10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTAACGGACCAGAG10.17667844522968199No Hit
CCCGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTA10.17667844522968199No Hit
CCAGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA10.17667844522968199No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTTTCGGCCCAGAG10.17667844522968199No Hit
CCTGTTCGCTACCCCAGCCTTCGCACCTCAGCGTCAGTTACAGGCCAGAA10.17667844522968199No Hit
ACTGTTTGCGCCCCACGCTGTCGAACCTCAGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCTGTTTGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAACCCAGAG10.17667844522968199No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAG10.17667844522968199No Hit
GAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGA10.17667844522968199No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC10.17667844522968199No Hit
CCTGTTCGCTACCCCCGCCTTCGCGCCTCAGCGTCAGTTACAGGCCAGAA10.17667844522968199No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAACAGCCCAGAG10.17667844522968199No Hit
CCTGTTCGCTACCCACACTTTCGAGCCTCAGCGTCAGTTACGCTCCCGTG10.17667844522968199No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA10.17667844522968199No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATGACCGTGTAGTG10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCCGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCTAGCG10.17667844522968199No Hit
CCTGTTCGCTCCCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGGTCCAGAA10.17667844522968199No Hit
CCCGTTTGCTCCCCTGGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA10.17667844522968199No Hit
CCCGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTTTTCCGCCGGAA10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCTGTTTGCTACCCACTCTTTCTCTCTTTAGCGTCAGTATCTGTCCAGTA10.17667844522968199No Hit
AATAGGGCAGTAGGAGAGCAGAATTTCAACTGTGGCCGGGGCGTAAAGAC10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAA10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTAATCGTCCAGAA10.17667844522968199No Hit
CCTGTTTGCTCCCCTCGCTTTCGAACCTCAGCGTCAGTTACCGTCCAGTG10.17667844522968199No Hit
GTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCAT10.17667844522968199No Hit
GTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATG10.17667844522968199No Hit
CCTGTTCGCTCCCCATGCTTTCGCGCCTCAGCGTCAGTTTCCGTCCAGAA10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCTGTTTGCTACCCACGCTTTCGATTCTCAGTGTCAATATTATGCCAGGA10.17667844522968199No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGACCAGTG10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGGGCATGAGCGTCAGTTTTATTCCTGGG10.17667844522968199No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC10.17667844522968199No Hit
ATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAA10.17667844522968199No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG10.17667844522968199No Hit
CTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGCTGAGG10.17667844522968199No Hit
CCCGTTCGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTATCAGCCCAGCA10.17667844522968199No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA10.17667844522968199No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGAA10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTTTTATCCCAGGG10.17667844522968199No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATGATGCCAGGG10.17667844522968199No Hit
CAAACTTTTTTTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCT10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTGTTATCCCAGGG10.17667844522968199No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTACCGCCCAGTA10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA10.17667844522968199No Hit
CCTGTTTGCTCCCCTCGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG10.17667844522968199No Hit
CCTGTTCGCTCCCCCCGCCTTCGCGCCTCAGCGTCGGTCTCGGCCCCGAC10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGTTTCAGTCCAGGT10.17667844522968199No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA10.17667844522968199No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGAG10.17667844522968199No Hit
CAGTGACCCGTCCGTGACTCGGGCCCACCATCTTGCTGGCCCAACCCTAA10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTGATATCCCAGGG10.17667844522968199No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAGCGTCAGTTTTATCCCAGGA10.17667844522968199No Hit
TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAA10.17667844522968199No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA10.17667844522968199No Hit
CCCTTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGTC10.17667844522968199No Hit
CCTGTTCGATCCCCACGCTTTCGCGCCTCAGCGTCTGTTACAGCCCAGAA10.17667844522968199No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTTCATTCCTGAC10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAACCAGAG10.17667844522968199No Hit
CCTGTTTGCTCCCCTCACTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCATGAGCGTCAGTTATATCCCAGGG10.17667844522968199No Hit
CCCCTGCAATTAAAATTGTTGACCACCTACATACCAAAGACGAGCGCCTT10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTCCAGACCAGAG10.17667844522968199No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGAGTCTCAGCGTCAGTTACAGCCCAGAG10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA10.17667844522968199No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC10.17667844522968199No Hit
CCTGTTTGCTCCCCTCGCTTTCGCGCTTCAGCGTCAGTTACAGTCCAGAG10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG10.17667844522968199No Hit
CCCATTTGCTCCCCTAGCTTTCTTCTCTCAGTTTCAGTGTCGGCTTCGCC10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA10.17667844522968199No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTCAGGCCCAGGC10.17667844522968199No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTTCTGTCCAGTA10.17667844522968199No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTTACGGACTAGAG10.17667844522968199No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACAGTCCAGAA10.17667844522968199No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA10.17667844522968199No Hit
CCTTTTTGCTTCCCACGCTTGCCTGCCTCCGCGTCATGTACAGTCCTGTC10.17667844522968199No Hit
CCTGTTCGATCCCCGCACTTTCGAGCCTCAGCGTCAGTTGTAGTCCCGAA10.17667844522968199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCG100.0022005576194.363659
GTTTGAT100.0022005576194.363654
CTCCCCA201.7189268E-7194.363659
TGATCCC100.0022005576194.363657
TGTTCGC100.0022005576194.363653
TGTTTGA100.0022005576194.363653
GCTACCC201.7189268E-7194.363658
GATCCCC100.0022005576194.363658
TTGATCC100.0022005576194.363656
GCTCCCC201.7189268E-7194.363658
CTACCCA100.0022005576194.363659
GTTCGCT100.0022005576194.363654
TTTGATC100.0022005576194.363655
GTTTGCT307.2759576E-12194.363634
CCTGTTT350.0194.363631
CCTGTTC152.0795724E-5194.363631
TGTTTGC251.2259989E-9194.363633
TGCTCCC152.0795724E-5194.363637
TGCTACC152.0795724E-5194.363637
CTGTTTG350.0194.363632
Produced by FastQC (version 0.11.5)