FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214
Sequences flagged as poor quality0
Sequence length198-201
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT23519.357495881383855No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG14511.943986820428336No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG746.095551894563426No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA453.706754530477759No Hit
CCCGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTCCGGCCCAGGG201.6474464579901154No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC201.6474464579901154No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG191.5650741350906094No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG171.400329489291598No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG171.400329489291598No Hit
CCTGTTTGATCCCCGCACTTTCGAGCCTCAGTGTCAGTTACAGTTTTGTG161.3179571663920924No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG151.2355848434925865No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGCTGGTCCAGGT141.1532125205930808No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG141.1532125205930808No Hit
CCTGTTTGATCCCCACGCTATCGTGCCTCAGCGTCGATCCGCCCCCAGCG131.070840197693575No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAACGGACCAGTA120.9884678747940692No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA110.9060955518945634No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG80.6589785831960462No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG80.6589785831960462No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCACAAATCGTCCAGGT80.6589785831960462No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGTGTCAGTATCTGTCCAGGG70.5766062602965404No Hit
CCTGTTTGCTCCCCACGCTATCGCGCCTCAGCGTCAGAAATGGACCAGGT70.5766062602965404No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG70.5766062602965404No Hit
CCTGTTTGCTACCCACGCTTTCGTACATGAGCGTCAGTGTCATCCCAGGG70.5766062602965404No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG70.5766062602965404No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA70.5766062602965404No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA60.4942339373970346No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACAGGCCCAGGG60.4942339373970346No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA60.4942339373970346No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC50.41186161449752884No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTAATGGCCCAGAG50.41186161449752884No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC50.41186161449752884No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGA50.41186161449752884No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGAGCCAGTA40.3294892915980231No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT40.3294892915980231No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG30.2471169686985173No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGG30.2471169686985173No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA30.2471169686985173No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA30.2471169686985173No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT30.2471169686985173No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTACAGGCCCAGGG30.2471169686985173No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG30.2471169686985173No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG30.2471169686985173No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG30.2471169686985173No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG30.2471169686985173No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA20.16474464579901155No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTGCAGGCCCAGGG20.16474464579901155No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCAGGCCCAGGG20.16474464579901155No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGCGGACCAGGT20.16474464579901155No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA20.16474464579901155No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGTGTCAGTATTAGGCCAGAT20.16474464579901155No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA20.16474464579901155No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCATCAGCGTCAATGTCGGTCCAGGA20.16474464579901155No Hit
CCTGTTTGATCCCCACGCTATCGCGCCTCAGCGTCGATCCGCCCCCAGCG20.16474464579901155No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGCGTCAGTGTTAGGCCAGAT20.16474464579901155No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGCGGACCAGGT20.16474464579901155No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG20.16474464579901155No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGA20.16474464579901155No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC20.16474464579901155No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA20.16474464579901155No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTCGTCCCAGGG20.16474464579901155No Hit
CCTGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTACCGTCCAGAA20.16474464579901155No Hit
CCTGTTTGCTCCCCACGCTTTTGCACCTGAGCGTCAGTCTTCGTCCAGGG20.16474464579901155No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAACGTCAGTGATATCCCAGAA20.16474464579901155No Hit
CCCGTTCGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTTTTCGTCCAGAA20.16474464579901155No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA20.16474464579901155No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA20.16474464579901155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAACCG108.695203E-4274.88235195
TCTCCGG108.695203E-4274.88235195
CAGGCCG400.0274.88235195
GTTTGAT100.002582294194.708344
CTCCCCA950.0194.708349
TGTTCGC100.002582294194.708343
TGTTTGA100.002582294194.708343
GGTTTGC400.0194.708343
TGATACC100.002582294194.708347
TTGATAC100.002582294194.708346
CGGTTTG400.0194.708342
GCTCCCC1050.0194.708348
CCGGTTT400.0194.708341
TTTGATA100.002582294194.708345
GTTTGCT900.0194.708334
CCTGTTT550.0194.708331
CCTGTTC152.9270515E-5194.708331
GATACCC152.9270515E-5194.708338
TGTTTGC450.0194.708333
TGCTCCC900.0194.708337
Produced by FastQC (version 0.11.5)