FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150728
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA9250861.37413088477257No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA2989719.835067140809937No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA141329.37582930842312No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA32632.1648267077119048No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA17261.1451090706438087No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA9190.6097075526776711No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA4060.2693593758293084No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA3050.20235125524122924No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA2520.16718857810095006No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA2370.15723687702351255No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1600.10615147815933336No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1520.10084390425136669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGA50.00522572031250.3804195
GCAGGCA50.00522572031250.3804195
GAAGCCG50.00522572031250.3804195
AAGATAG152.2289896E-81250.3804195
AGGCAGG700.01250.3804195
AAGCGGG2050.01189.3861195
AAGGCGG4050.01173.1964195
GAAGGCA16000.01113.62195
AAGGCAG1800.0520.9918195
AAGCTGG250.0043392307250.07607195
AAGGGGG1200.0208.39673195
GGTCCCG128800.0194.15337
GTATGTC308.54925E-11194.153294
GAGGTCC204.9651317E-7194.153295
TCCCAAG153.8360995E-5194.153299
GTCACGA100.0029589727194.153298
TAGTGCG100.0029589727194.153295
TACAGAG100.0029589727194.153291
TGCGCGA100.0029589727194.153298
GTCGCGA750.0194.153298
Produced by FastQC (version 0.11.5)