FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684
Sequences flagged as poor quality0
Sequence length199-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCGCACTTTCGAGCCTCAGTGTCAGTTACAGTTTTGTG1176.947743467933491No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1015.997624703087886No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG804.750593824228028No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG563.32541567695962No Hit
CCTGTTTGATCCCCGCACTCTCGTGCCTGAGCGTCAATACTGGCCCAGCC492.9097387173396676No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG432.553444180522565No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT422.494061757719715No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG412.434679334916865No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA362.137767220902613No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG352.0783847980997625No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA342.019002375296912No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA311.840855106888361No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA261.5439429928741093No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT261.5439429928741093No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG251.4845605700712587No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA241.4251781472684086No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA241.4251781472684086No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG231.3657957244655583No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA231.3657957244655583No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA231.3657957244655583No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG221.3064133016627077No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGGCCAGTG221.3064133016627077No Hit
CCTGTTCGCTACCCACGCTTTCGTTCCTCAGCGTCAGTTACTGCCCAGAG211.2470308788598574No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTCGTCCCAGGG211.2470308788598574No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA201.187648456057007No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGTGTCAGTATCTGTCCAGGG181.0688836104513064No Hit
CCGGTTTGCTCCCCTAGCTTTCGTCCCTCACCGTCGGATCTGTTCTCGTC181.0688836104513064No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA181.0688836104513064No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG160.9501187648456058No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG160.9501187648456058No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG150.8907363420427553No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA150.8907363420427553No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA140.831353919239905No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG140.831353919239905No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCTGTCCAGTG130.7719714964370546No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCAGGCCAGAG130.7719714964370546No Hit
CCTGTTTGCTCCCCACGCTTTCGTATCTCAGCGTCAATGTAGGACCAGGC130.7719714964370546No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA120.7125890736342043No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA120.7125890736342043No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACCGGGCCAGTG100.5938242280285035No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACCAGCCTAGTC100.5938242280285035No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG100.5938242280285035No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAATACCAGACCAGTA100.5938242280285035No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTAGTGGCCCAGAG90.5344418052256532No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAA90.5344418052256532No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA80.4750593824228029No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG80.4750593824228029No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTGTCAGTCCAGGT80.4750593824228029No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA80.4750593824228029No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA70.4156769596199525No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG70.4156769596199525No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTACCCAGAG70.4156769596199525No Hit
CCTGTTTGCTACCCACGCTTTCGAATCTCAGTGTCAATATTATGCCAGGA70.4156769596199525No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG60.35629453681710216No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG60.35629453681710216No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATCTTGACCCAGGG60.35629453681710216No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG60.35629453681710216No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC50.29691211401425177No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG50.29691211401425177No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG40.23752969121140144No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGCGGGCCCAGCG40.23752969121140144No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA40.23752969121140144No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA30.17814726840855108No Hit
CCTGTTCGCTACCCACGCTTTCGTCCATCAGCGTCAGTTAAATCTTAGTG30.17814726840855108No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTATCCCAGGG30.17814726840855108No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG30.17814726840855108No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA30.17814726840855108No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTCAGGCCCAGGC30.17814726840855108No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA30.17814726840855108No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC30.17814726840855108No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTATTCGTCCAGAA20.11876484560570072No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCCGGCCCAGGG20.11876484560570072No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGAA20.11876484560570072No Hit
CCTGTTTGATCCCCGCACTTTCGCGCCTCAGTGTCAGTTACAGTTTTGTG20.11876484560570072No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC20.11876484560570072No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG20.11876484560570072No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGCGCCAGTG20.11876484560570072No Hit
CCTGTTTTCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT20.11876484560570072No Hit
CCCGTTCGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTTTTCGTCCAGAA20.11876484560570072No Hit
CCTGTTCGCTCCCCTTGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG20.11876484560570072No Hit
GCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG20.11876484560570072No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG20.11876484560570072No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGTG20.11876484560570072No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG20.11876484560570072No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA20.11876484560570072No Hit
CCTGCTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTCGTCCCAGGG20.11876484560570072No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG20.11876484560570072No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGCTGCCCAGTA20.11876484560570072No Hit
CCTGTTTGATCCCCGCACTTTCGAGCCTCAGTGTCAGTTGCAGTTTTGTG20.11876484560570072No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG20.11876484560570072No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGCGTTGGTA20.11876484560570072No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA20.11876484560570072No Hit
CCTGTTTGATCCCCGCACTTTCGAGCCTCAGTGTCACTTACAGTTTTGTG20.11876484560570072No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG20.11876484560570072No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA20.11876484560570072No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG20.11876484560570072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG151.157615E-5246.96153195
CATGCCG201.0001531E-7246.96153195
TGTTTGC1100.0194.575783
TGCTCCC1100.0194.575787
TTTGCTC1100.0194.575785
TTGCTCC1100.0194.575786
ATCCCCG100.002682458194.575769
GTTTGCT1200.0194.575764
GTTTGAT100.002682458194.575764
CCTGTTT1200.0194.575761
CCTGTTC304.1836756E-11194.575761
CTCCCCA1150.0194.575769
TGATCCC100.002682458194.575767
GGTTTGC100.002682458194.575763
GCTACCC100.002682458194.575768
GATCCCC100.002682458194.575768
TTGATCC100.002682458194.575766
TGCTACC100.002682458194.575767
CTGTTTG1200.0194.575762
CGGTTTG100.002682458194.575762
Produced by FastQC (version 0.11.5)