FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125459
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2723021.70430180377653No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1622412.931714743462008No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA1480411.79987087415012No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA104148.300719757052105No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA84596.742441753879754No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA83596.66273443913948No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA71555.703058369666585No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA63205.037502291585299No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA59584.748961812225508No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA41573.313433073753178No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA39343.1356857618823684No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA31572.5163599263504413No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA18161.4474848356833707No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA2360.18810926278704596No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA1860.14825560541690913No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA1390.11079316748898045No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1380.10999609434157773No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGAGCGCA1360.10840194804677225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCAG203.2705975E-7211.07596195
AAGGCAG52000.0210.06119195
AAGGCGG46650.0208.13493195
AAGGCGA16200.0207.16716195
ACAGAGG29800.0194.923482
TACAGAG29850.0194.923481
ACGTCGG256.3046173E-9194.923482
AGGATCC12200.0194.923486
GGTCCCG8750.0194.923487
TACGGAG18650.0194.923481
TAGCAAG153.7745107E-5194.923489
CAGAGGG29800.0194.923483
GTCCCGA8750.0194.923488
TACGTCG256.3046173E-9194.923481
GATCCGA12200.0194.923488
GTAGGTC8750.0194.923484
CGTCGGT256.3046173E-9194.923483
TCCCGAG8800.0194.923489
GTGCGAG18150.0194.923489
GGGCGAG4750.0194.923489
Produced by FastQC (version 0.11.5)