FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005301353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3311.073825503355705No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA165.369127516778524No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA144.697986577181208No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA124.026845637583892No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA82.684563758389262No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA72.348993288590604No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA72.348993288590604No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA62.013422818791946No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA62.013422818791946No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA62.013422818791946No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA62.013422818791946No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA51.6778523489932886No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA51.6778523489932886No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA51.6778523489932886No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA41.342281879194631No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA41.342281879194631No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA41.342281879194631No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA41.342281879194631No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA31.006711409395973No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA31.006711409395973No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA31.006711409395973No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA31.006711409395973No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA31.006711409395973No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA31.006711409395973No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA20.6711409395973155No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA20.6711409395973155No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA20.6711409395973155No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA20.6711409395973155No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA20.6711409395973155No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA20.6711409395973155No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA20.6711409395973155No Hit
CCTGATTCAGCGAAACCAATCCGCGGCATTTAGTAGCGGTAAAGTTAGAC20.6711409395973155No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATAATTGGGCGTAAAGGGCGCGTA20.6711409395973155No Hit
TATCAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGTAAAACAGGT10.33557046979865773No Hit
TACGGAGGGTGCGAGCGTTATTCGGAATAACTGGGCGTAAAGGGCACGCA10.33557046979865773No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGTGCATGTA10.33557046979865773No Hit
GATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGG10.33557046979865773No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA10.33557046979865773No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA10.33557046979865773No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA10.33557046979865773No Hit
GCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTT10.33557046979865773No Hit
AACGTAGGTCACAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA10.33557046979865773No Hit
ATCAGTGACGACATTAGAAATATCCTTTGCAGTAGCGCCAATATGAGATG10.33557046979865773No Hit
CACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGCGAT10.33557046979865773No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGGGCTCGCA10.33557046979865773No Hit
TTAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGCTGAGACAGGCCGT10.33557046979865773No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA10.33557046979865773No Hit
CGAATCACCAGAACGGAAAACATCCTTCATAGCAATTTCACGCGGCGGCA10.33557046979865773No Hit
ACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTGAGCATAAGACTGAGCCA10.33557046979865773No Hit
ACCAAACATACATCACCTCACTTAAGTGGCTGGAGACAAATAATCTCTTT10.33557046979865773No Hit
TACGTAGGTCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA10.33557046979865773No Hit
AATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAATTCCA10.33557046979865773No Hit
TACGTAGGTCCCGAGCGTTGTCCGTATTTATTGGGCGTAAAGCGAGCGCA10.33557046979865773No Hit
TTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGG10.33557046979865773No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCTCGTA10.33557046979865773No Hit
GCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGT10.33557046979865773No Hit
CAGCAGAGGAAGCATCAGCACCAGCACGCTCCCACGCATTAAGCTCAGGA10.33557046979865773No Hit
TACGTAGGTGCCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA10.33557046979865773No Hit
ATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACT10.33557046979865773No Hit
CCAAATCAAGCAACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTA10.33557046979865773No Hit
ATGGAAGCGATAAAACTCTGCAGGTTTTATACGCCAATCATTTTTATCGA10.33557046979865773No Hit
TACGGATGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.33557046979865773No Hit
AACAGGCAAAAAATTTAGGGTCGGCATCAAAAGCAATATCAGCACCAACA10.33557046979865773No Hit
AATCTCTTTAATAACCTGATTCAGCGAAACCAATCCGCGGCATTTAGTAG10.33557046979865773No Hit
TCTGACTTTTTGTTAACGTATTTAGCCACATAGAAACCAACAGCCATATA10.33557046979865773No Hit
TGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCC10.33557046979865773No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTTCTGGGTGTAAAGGGAGCGTA10.33557046979865773No Hit
CACGCGTATCCTTTCCTTTATCAGCGTCATACTTGCCACCATGTCCCACA10.33557046979865773No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTGCGTA10.33557046979865773No Hit
GTCGTGGCCTTGCTATTGACTCTACTGTCGACATTTTTACTTTTTATGTC10.33557046979865773No Hit
CTTATCAGAAACGGCAGAAGTGCCATCCTGCAACGTACCTTCAAGAAGTC10.33557046979865773No Hit
CCAAGCAACAGCAGGTTTCCGAGATTACGCGCCAAATGCTTACTCAAGCT10.33557046979865773No Hit
ACCGGAGGCGGCTTTTTGACCGCCTCCAAACAATTTAGACATGGCGCCAC10.33557046979865773No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA10.33557046979865773No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCGCGCA10.33557046979865773No Hit
GTTCCTGAGCATGGTACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCC10.33557046979865773No Hit
TCAAACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTC10.33557046979865773No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGCGCGCGCA10.33557046979865773No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCGCGCA10.33557046979865773No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGTA10.33557046979865773No Hit
TACGTAGGTCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCTCGCA10.33557046979865773No Hit
TACGGAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.33557046979865773No Hit
TACGGAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.33557046979865773No Hit
TTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTA10.33557046979865773No Hit
ATCATCTTGATTAAGCTCATTAGGGATAGCCTCGGTACGGTCAGTCATCC10.33557046979865773No Hit
TCCACTGCAACAACTGAACGGACTGGAAACACTGGTCCTAATCATGGTGG10.33557046979865773No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA10.33557046979865773No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCCCA10.33557046979865773No Hit
TACGGAGGTTGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.33557046979865773No Hit
CCCGGCGTACGGGGAAGGACGCCAATATTCACACAGTCCTTGACGGTATA10.33557046979865773No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA10.33557046979865773No Hit
TACGTAGGTTGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGCGTA10.33557046979865773No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.33557046979865773No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA10.33557046979865773No Hit
TCCGGCTGACTGACTGTCGAAGAATCTCGTATGCCGTCTTCTGCTTGAAA10.33557046979865773Illumina PCR Primer Index 8 (96% over 27bp)
TACGTAGGTCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.33557046979865773No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAACGGGCATCTA10.33557046979865773No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA10.33557046979865773No Hit
ATAAACGTGACGATGAGGGACATAAAAAGTAAAAATGTCTACAGTAGAAT10.33557046979865773No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.33557046979865773No Hit
TACATAGGGGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGCGTA10.33557046979865773No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10.33557046979865773No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTCAAGGGAGCGTA10.33557046979865773No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA10.33557046979865773No Hit
TACGTATGTCGCCAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA10.33557046979865773No Hit
ATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAG10.33557046979865773No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA10.33557046979865773No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGAGCGTA10.33557046979865773No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCATCCA10.33557046979865773No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGATTGCA10.33557046979865773No Hit
CCAGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCTGCACGTAAT10.33557046979865773No Hit
TACGTAGGGGGCGAGCGTTATCCTGATTTACTGGGTGTAAAGGGAGCGTA10.33557046979865773No Hit
AATTGCAGGTGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATT10.33557046979865773No Hit
TTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTACCGCTTCG10.33557046979865773No Hit
TGCAGTAGCGCCAATATGAGAAGAGCCATACTGCTGATTCTGCGTTTGCT10.33557046979865773No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.33557046979865773No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTCTTGGGTTTACAGGGAGCGTA10.33557046979865773No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGCCGTAAAGGGCACGCA10.33557046979865773No Hit
TATGTGGCTAAATACGTTAACAAAAATTCAGATATGGACCTTGCTGCTAA10.33557046979865773No Hit
ACTAAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAGAA10.33557046979865773No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGATCGCA10.33557046979865773No Hit
TTGAGTAAGCATTTGGCGCATAATTTCGGAAACCTGCTGTTGCTTGGAAA10.33557046979865773No Hit
TGTCGCTACTTCCCAAGCCGCTGTTCACAATCAGAATGAGCCGCAACTTC10.33557046979865773No Hit
GTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTT10.33557046979865773No Hit
GTTCTCTAAAAACCATTTTTCGTCCTCTTCGGGGCGGTGGTCTATAGTGT10.33557046979865773No Hit
ATAAACGCAAGCCTCAACGCAGCGACGAGCACGCGAGCGGTCAGTAGCAA10.33557046979865773No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA10.33557046979865773No Hit
TACGTAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCTCGCA10.33557046979865773No Hit
TCAAACTATCAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGTAAA10.33557046979865773No Hit
ATTCCTTGACGGTATAATAACCACCATCATGGCGACCATCCAAAGGATAA10.33557046979865773No Hit
TCCGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTATCGCGATCGCA10.33557046979865773No Hit
TTAGACATTACATCACTCCTTCTGCACGTAATTTTTGACGCACGTTTTCT10.33557046979865773No Hit
TTTGGATTTAACCGAAGATGATTTCGATTTTCTGACGAGTAACAAAGTTT10.33557046979865773No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA10.33557046979865773No Hit
ATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGATTTAATACCAGCAT10.33557046979865773No Hit
GCCATAGCACCAGAAACAAAACTAGGGACGGCCTCATCAGGGTTAGGAAC10.33557046979865773No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGTGCA10.33557046979865773No Hit
GTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGT10.33557046979865773No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGTA10.33557046979865773No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTTCGTA10.33557046979865773No Hit
TACGGAGGGTGCAAGCGTTATTCGGATTTACTGGGCGTAAAGCGCACGTA10.33557046979865773No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA10.33557046979865773No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA10.33557046979865773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)