FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151257
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3694224.423332473868978No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2381015.741420231790926No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1804611.930687505371653No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1543910.207130909643851No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA78345.179264430737089No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA49983.30430988317896No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA40322.6656617544973127No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA39892.6372333181274255No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA37772.4970745155596106No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA28921.911977627481703No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA26391.7447126413984146No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA23391.546374713236412No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA22611.4948068519142916No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA19301.275974004508882No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA17841.1794495461367076No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA14900.9850783765379454No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA12810.8469029532517504No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA9040.597658290194834No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA8710.5758411180970137No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA8090.5348512796101998No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA5140.3398189835842308No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA4840.31998519076803056No Hit
TACGGAAGGTTCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA3170.20957707742451587No Hit
TACGCATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA3080.2036269395796558No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2740.18114864105462888No Hit
TACGCAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA2440.16131484823842865No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2330.15404245753915521No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA2220.14677006683988178No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1810.11966388332440812No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA1660.109746986916308No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTCGTA1610.10644135478027462No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1600.10578022835306797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCAT202.5405825E-8352.1939195
AAGATAG26350.0350.85727195
AAGGCAG33050.0333.54517195
AAGCGGG1500.0328.7143195
AAGCTAG550.0288.15866195
AAGCCGG257.7348886E-8281.75513195
AAGGCGG14250.0263.2186195
AGGCAGG2400.0256.80807195
GTATGTG100.0029409316194.550754
GGATGCG500.0194.550757
GAGCGAG256.382834E-9194.550759
GTGTCAA100.0029409316194.550758
CGGAGGA500.0194.550753
GTCACAA2750.0194.550758
TAGTGCG100.0029409316194.550755
ACGCATG308.367351E-11194.550752
AGGATGC500.0194.550756
GCAGGTC400.0194.550754
GGTCCAG15600.0194.550758
GGTCCAA100.0029409316194.550758
Produced by FastQC (version 0.11.5)