FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429
Sequences flagged as poor quality0
Sequence length200-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG15135.1981351981352No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG337.6923076923076925No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATTAAACCATAGTA194.428904428904429No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT173.9627039627039626No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA163.7296037296037294No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA153.4965034965034967No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATCTTGACCCAGGG133.0303030303030303No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG102.331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG81.8648018648018647No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTGTTATCCCAGGG71.6317016317016315No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG71.6317016317016315No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGTCCAGGG51.1655011655011656No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA51.1655011655011656No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG40.9324009324009324No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG40.9324009324009324No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGAAACGGACCAGCT40.9324009324009324No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAGCGTCAGTGTTATCCCAGGG30.6993006993006993No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG30.6993006993006993No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATAACTGTCCAGTG30.6993006993006993No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG20.4662004662004662No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA20.4662004662004662No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA20.4662004662004662No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTATTCGCCCAGAC10.2331002331002331No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTCTTGTCCCAGAG10.2331002331002331No Hit
GACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGA10.2331002331002331No Hit
CCTGTTCGCTCCCCTAGCTTTCGCTTCTCAGCGTCAGTTACCGCCCAGTG10.2331002331002331No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC10.2331002331002331No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG10.2331002331002331No Hit
CCTGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTATAGACCAGAG10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATAGCCCAGAG10.2331002331002331No Hit
CCTGTTTGCTCCCCTCGCTTTCGCGCCTCCGCGTCAGTTTTCGTCCAGAA10.2331002331002331No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCCTCCCGAA10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGCGTCAGTGTTATCCCAGGG10.2331002331002331No Hit
TCAGTTTGATCCGCACGCTCACGCACACGGGAGTCAGTTACAGTCGAGAA10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCATCGTCAGTAACGTCCCACTC10.2331002331002331No Hit
CCTGTTTTCTCCCCCCACCTTCGCTCATCACCTTCATTTTTCGTCCTTTA10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCCGTACATTCCCAAGG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACTAGAC10.2331002331002331No Hit
CCCGTTCGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCTAGTA10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCACGCCTTAGCGTCAGTTACGTTCTAGCA10.2331002331002331No Hit
CCTGTTTGCTACCCACACTTTCGCACCTCAGCGTCAGTATCATTCCAGGC10.2331002331002331No Hit
CCTGTTTGATACCCACGCTTTCGTTCTTCAGCGTCAGTTACGGCTTAGAA10.2331002331002331No Hit
CCGGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGATATAGACCAGTA10.2331002331002331No Hit
CCTGTTTGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTTTCCGTCCAGTG10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCGCACATCAGCGTCAGTATGAGGCCAGGG10.2331002331002331No Hit
CCTGTTTGATCCCCACGCTTTCGCACTTCAGCGTCAGTTACAGTCCAGTA10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCTATCCCAGTG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAATCCAGAG10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG10.2331002331002331No Hit
CCTGTTTGATACCCTCACCTTCGCGCCTCAGCGTCAGTTTTCCTCCCGTA10.2331002331002331No Hit
CCGGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGAAACGGCTCAGTA10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGC10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCCTTCGTGCATGAGCGTCAGTGTTATCCCAGGG10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA10.2331002331002331No Hit
CCTGTTTGATACCCACGCTTTCGCACATCAGCGTCAGTTACATACCAGAA10.2331002331002331No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTAAACCATAGTA10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGAACATTCCCAATG10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCTTCAGCGTCAGTTACAGTCCAGTC10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGTG10.2331002331002331No Hit
CCTGTTCGATCCCCACGCTTTCGTTCTTCAGCGTCAGTTACGGCTTAGTG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCTCACATGAGCGTCAGTACATTCCCAAGG10.2331002331002331No Hit
CCCGTTCGCTCCCCTAGCTTTCGATCCTCAGCGTCAGTTACAGTCCAGTC10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTATCCCAGGG10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG10.2331002331002331No Hit
CCCGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCATGG10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGTG10.2331002331002331No Hit
GGCCTCCGAGCCCCTGCTCAGACACCCGTTCTCTCCTCATTCTTCCCTCC10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAG10.2331002331002331No Hit
CCTGTTTGCTACCCAAGCTTTCGCGCTTCAGCGTCAGTTACAGACCAGAC10.2331002331002331No Hit
TCTTTTTGCGTTCTGCTTCACTATCTGGTTGAACGGCGTCGCGTCGTAAC10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAACCCAGAG10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCCCTCCTCAGCGTCAGTAACGGCTTAGTA10.2331002331002331No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATTACACCATAGTA10.2331002331002331No Hit
CCCGTTTGATCCCCTGGCTTTCGCACCTGAGCGTCAGTCCTCGTCCAGGG10.2331002331002331No Hit
CCTGTTTGCTACCCACACTTTCGATCCTCAGCGTCAGTTGCTGCCCAGTA10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA10.2331002331002331No Hit
CCTGTTTGCTACCCACGCCTTCGAGCCTCAGCGTCAGTTACGCTCCAGTC10.2331002331002331No Hit
CCTGTTTGATACCCACACTTTCGAGCTTCAGCGTCAGTTGCGGTCCCGTC10.2331002331002331No Hit
CCTGTTCGATACCCGAACTTTCGAACTTCAGCGTCAGTTGCGCTCCAGTC10.2331002331002331No Hit
CCTGTTCGATCCCCGCACCTTCGAGCCTAAGCGTCAGTTACGCTCCCGTG10.2331002331002331No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATAGACCAGAG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGGGCATGAGCGTCAGTGTTATCCCAGGC10.2331002331002331No Hit
CCTGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTCCGTCCAGTA10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGGCCAGAC10.2331002331002331No Hit
ACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGC10.2331002331002331No Hit
AGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCGGCTT10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGCCCAGAG10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCGCACTTCAGTGTCAGTATGATGCCAGGG10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCGCACATGAGCGTCAGTGCATTCCCAGGA10.2331002331002331No Hit
CCTGTTTGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTACCATCCAGAG10.2331002331002331No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA10.2331002331002331No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTTTTATCCCAGGA10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAATCCAGAG10.2331002331002331No Hit
CCTGTTCGCTCCCCACTCTTTCGCTCCTCAGCGTCAGTACCGGTCCAGTA10.2331002331002331No Hit
CGTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG10.2331002331002331No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG10.2331002331002331No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTATCGTCCAGTA10.2331002331002331No Hit
ACTGATTGCTCCCCACGCTTTCGAGCATGAGCGTCAGTGTTATCCCAGGG10.2331002331002331No Hit
CCTGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTTCATTCCCAATG10.2331002331002331No Hit
CCTGTTTGCTCCCCAGGCTTTCGAGCCTCAGCGTCAGTTTTAGACCAGAA10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGCTCCAGTC10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGAACCTCAGCGTCAGTTACCTGCCAGTG10.2331002331002331No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATTCGCCCAGAG10.2331002331002331No Hit
CCTGTTCGCTCCCCCCGCTTTCGCCCTTCAGCGTCAGTTACAGTCCAGTG10.2331002331002331No Hit
CCTGTTCGATCCCCTCGCTTTCGCGCATCAGCGTCAGTGATCGTTTAGAA10.2331002331002331No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG10.2331002331002331No Hit
CCTGTTCGCTCCCCACACTTTCGCTCCTCAGCGTCAGTTGCAGCCCGGAC10.2331002331002331No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTACCGCCCAGTA10.2331002331002331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAACCG101.390344E-4389.0195
GTTTGCT350.0194.54
CCTGTTT350.0194.51
CTCCCCA255.020411E-10194.59
TGTTTGC350.0194.53
GCTACCC100.001938988194.58
TGCTCCC255.020411E-10194.57
TGCTACC100.001938988194.57
CTGTTTG350.0194.52
GCTCCCC301.8189894E-12194.58
TTTGCTC255.020411E-10194.55
TTTGCTA100.001938988194.55
CTACCCA100.001938988194.59
TTGCTCC255.020411E-10194.56
TTGCTAC100.001938988194.56
CGTCAGT351.8596802E-738.930-34
TTCACAT256.304389E-538.9175-179
TTTCACA256.304389E-538.9175-179
CCTTCGC351.8596802E-738.955-59
GCGTCAG351.8596802E-738.930-34
Produced by FastQC (version 0.11.5)