FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75967
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2090627.5198441428515No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1392918.335593086471757No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1360317.906459383679756No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA73999.739755420116628No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA64308.464201561204208No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA40125.281240538654942No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA32804.317664248950202No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3020.39754103755578085No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA2350.30934484710466387No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA2340.30802848605315464No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGGGCACGCA2210.290915792383535No Hit
TACGGGGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1700.22378137875656537No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1600.21061776824147327No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1580.20798504613845484No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA1420.18692326931430753No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1400.1842905472112891No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA1230.16191240933563256No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGCGAGCGCA1210.15927968723261418No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGAGCTTGTA1140.15006515987204969No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1120.1474324377690313No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1070.14085063251148525No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCACGCA980.12900338304790238No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA930.12242157779035634No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA900.11847249463582872No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA860.11320705042979187No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA830.10925796727526427No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA790.10399252306922742No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA770.101359800966209No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA760.10004343991469981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG104.1743214E-4373.0745195
AAGGCAG23250.0369.06293195
AAGGCGG2400.0365.3021195
AAGCGGG1300.0358.7255195
AGGCAGG208.87699E-6279.80588195
GGATGCG351.8189894E-12194.519447
CGGAGGA351.8189894E-12194.519443
GAGGATG351.8189894E-12194.519445
ACAGAGG100.0029394766194.519442
TACAGAG100.0029394766194.519441
AGGATGC351.8189894E-12194.519446
GTCGCGA204.902813E-7194.519448
TATGTCG100.0029394766194.519445
TACGGAA204.902813E-7194.519441
GATGCGA351.8189894E-12194.519448
TCGCGAG204.902813E-7194.519449
ATGCGAG351.8189894E-12194.519449
CGTATGT204.902813E-7194.519443
AAGGTCC204.902813E-7194.519446
ATGTCGC100.0029394766194.519446
Produced by FastQC (version 0.11.5)