FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97691
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1809718.524736157885577No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1247612.770879610199506No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA1124311.508736731121598No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA88619.070436375919993No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA81408.332395000562999No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA42884.389350093662671No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA40904.186670215270598No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA32413.3176034639833762No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA29543.0238200038898158No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA22762.329794965759384No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA20422.090264200386934No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA16571.6961644368467925No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA13681.4003337052543223No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA11421.1689920258775117No Hit
TACGGAGGATGCGAGCGTTATTCGGAATCATTGGGTTTAAAGGGTCTGTA10541.0789120799254794No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA8820.9028467310192341No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA7630.7810340768340994No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA7380.7554431830977265No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA6780.6940250381304316No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA5580.571188748195842No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGTGCGTCTA5530.5660705694485674No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5320.5445742187100142No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA5120.5241015037209159No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA4730.4841797094921743No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA3700.37874522729831817No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA3640.3726034128015887No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA3450.3531543335619453No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA3190.32653980407611755No Hit
TACGTATGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGTA2370.2426016726208146No Hit
TACGTAGGTCTCAAGCGTTATCCGGATTTACTGGGCGTAAAGTGTCCGTA1600.16378171991278623No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1560.1596871769149666No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGGGCACGCA1540.15763990541605677No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1470.15047445516987235No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1260.12897810443131916No Hit
TACATATGGGGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1260.12897810443131916No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA1210.1238599256840446No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1190.12181265418513476No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1150.1177181111873151No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA1120.11464720393895035No Hit
CACGTAAGGGGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCATGTA1020.10441084644440123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCAT1400.0376.5015195
AAAGCAG152.7182177E-6376.5015195
AAGATAG5950.0373.33762195
AAGGCAG22700.0369.8671195
AAGCGGG500.0338.85135195
AAGGCGG14550.0332.51163195
GAGCGAG153.8035578E-5194.516149
TCCCAAG153.8035578E-5194.516149
CGGAGGA6350.0194.516143
GAGGATG6450.0194.516145
TGTCCCA153.8035578E-5194.516147
GGTCCCG20850.0194.516147
TATGTCC153.8035578E-5194.516145
GGTCTCA153.8035578E-5194.516147
TACGGAT153.8035578E-5194.516141
AGAGGGT153.8035578E-5194.516144
GTCCCGA20850.0194.516148
GTCCCAA153.8035578E-5194.516148
GTCTCAA153.8035578E-5194.516148
AGGTCTC153.8035578E-5194.516146
Produced by FastQC (version 0.11.5)