FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77330
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2525932.66390792706582No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG1011213.076425708004654No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT952312.314754946333894No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG71909.297814560972457No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA45095.830854778223199No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG34654.480796586059744No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG25733.3272985904564853No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG22522.9121944911418596No Hit
CCTGTTCGCTACCCATGCCTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT14301.849217638691323No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAC13471.7418854260959526No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG9421.2181559549980603No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3600.4655373076425708No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3440.44484676063623435No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGAG2770.35820509504720033No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2420.3129445234708393No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1940.2508728824518298No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTACAGACCAGAG1830.2366481313849735No Hit
CCTGTTCGCTACCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1700.21983706194232508No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1690.21854390275442906No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA1570.2030259924996767No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA1500.19397387818440448No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG1490.19268071899650846No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGGT1440.1862149230570283No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1320.17069701280227598No Hit
CCTGTTCGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTGCAGACCAGAG1040.1344885555411871No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGACCAGAG910.11767748609853873No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG900.1163843269106427No Hit
CCTGTTCGCTACCCATGCTTTCGCGTCTCAGCGTCAGTTGCAGACCAGGT810.10474589421957843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG3750.0503.38358195
TTAACCG158.485313E-7503.38358195
TGTACCG101.6989812E-4503.38358195
GGAACCG300.0503.38358195
AAAACCG10900.0501.0745195
TGAACCG9950.0500.85403195
CGAACCG2400.0492.89642195
GCAACCG1500.0486.60413195
TCTCCAG350.0431.47162195
CGTTCGC4700.0194.385533
ATCCCCA100.0029456413194.385519
CGATACC450.0194.385517
CCTGTTC64350.0194.385511
CTCCCCA41200.0194.385519
GTTCTCT100.0029456413194.385514
TGATCGC153.8106984E-5194.385513
TGTTCGC63600.0194.385513
CCTGATC153.8106984E-5194.385511
TGTTCAC100.0029456413194.385513
GATCGCT204.920239E-7194.385514
Produced by FastQC (version 0.11.5)