FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151793
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3285721.645925701448686No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2771418.25775892168941No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA126198.313295079483243No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA124308.188783408984603No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA116217.6558207558978335No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA103376.809931946795967No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA57833.809793600495411No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA51893.418471207499687No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA39442.598275282786426No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA25881.7049534563517421No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA24651.6239220517415165No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA24181.5929588320937067No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA17911.179896306153775No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA11720.7721041154730456No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA10760.7088600923626254No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA9650.6357341906412022No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA9280.6113588900673944No Hit
TACGCAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA8560.5639258727345793No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA8080.5323038611793692No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA5470.3603591733479146No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA5260.3465245432925102No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA5220.3438893756629094No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA4660.3069970288484976No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA4350.2865744797190911No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA3680.2424354219232771No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3200.21081341036806703No Hit
TACGCATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA3010.19829636412746304No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA2490.16403918494265216No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2410.15876884968345048No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1990.1310995895726417No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGAGCATCTA1560.10277153755443268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCAT103.818305E-4384.57657195
AGATAGT103.818305E-4384.57657195
AAGATAG16400.0382.2316195
AAGGCAG45050.0381.5887195
AGGCAGG207.892506E-6288.43243195
AAGCGGG1200.0288.4324195
AGGCGAG150.0012861729256.38437195
CAGGCAG150.0012861729256.38437195
AAGGCGG11000.0209.76901195
GGTTGCA100.002942976194.505787
GTCACAA100.002942976194.505788
ACGCATG450.0194.505782
GTCGCGA5500.0194.505788
TATGTCA100.002942976194.505785
TACGGCA100.002942976194.505781
TCGCGAG5500.0194.505789
TGTCACA100.002942976194.505787
CGTAGGA204.9205664E-7194.505783
TCCAGAG100.002942976194.505789
TACGCAT450.0194.505781
Produced by FastQC (version 0.11.5)