FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85973
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2081324.208763216358626No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1814521.105463343142613No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1251514.556895769602086No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1078212.541146639061102No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA76018.841147802216975No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA26163.0428157677410352No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA23542.738068928617124No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA23372.7182952787503054No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA8500.9886824933409326No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA6460.7513986949391088No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA6170.7176671745780653No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5070.5897200283810033No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA4670.5431937934002535No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA3690.4292045176974167No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGGGCACGCA2450.2849731892570923No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2100.2442627336489363No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA2060.2396101101508613No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1200.1395787049422493No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA870.10119456108313074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCAGG550.0418.3792195
CAGGCAG102.9611815E-4418.3792195
AAGGCAG30100.0407.9545195
AAGGCGG2750.0403.16544195
AAGCGGG600.0383.51428195
GGATGCG650.0194.464817
GAGGATG550.0194.464815
GGCGCGA3550.0194.464818
GGTCCCG28850.0194.464817
TACGGAG13350.0194.464811
GATGCGA650.0194.464818
GCGCGAG3550.0194.464819
ATGCGAG650.0194.464819
GTCCCGA28850.0194.464818
AAGGTCC16500.0194.464816
AGGTCCG13850.0194.464817
GTAGGTC29100.0194.464814
GGAGGAT550.0194.464814
GTGGCAA21500.0194.464818
TCCCGAG28850.0194.464819
Produced by FastQC (version 0.11.5)