FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85973
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1865421.697509683272656No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1671719.44447675432985No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1127113.109929861700767No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1016311.821153152733997No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG72968.486385260488758No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG26443.07538413222756No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG22732.6438533027811055No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA14291.6621497446872857No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG12851.4946552987565864No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA12381.4399869726542054No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG9651.1224454189105881No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC8500.9886824933409326No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA8210.9549509729798891No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA5950.6920777453386528No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA5040.5862305607574471No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC4850.5641305991415909No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA4760.5536621962709223No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC3060.35592569760273574No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG2230.25938376001768No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG2030.2361206425273051No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1730.20122596629174277No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC1340.15586288718551172No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG1200.1395787049422493No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1140.13259976969513684No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCACGG1070.12445767857350563No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG880.10235771695764949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACCG203.445166E-9524.52594195
TAATCCG300.0524.52594195
TCACCAG101.5023592E-4524.52594195
CAGGCCG600.0524.52594195
GTAACCG157.203653E-7524.52594195
GTCACCG9000.0524.5259195
CCAGCCG450.0524.5259195
TCTCCAG12650.0524.5259195
GGAACCG2250.0524.5259195
CAAACGG550.0524.5259195
TAAACCA1250.0503.54483195
TCTCCGG1100.0476.8417195
CCAACCG255.541562E-6314.7155195
TCCACGG2500.0251.77242195
CGTTCGC18150.0194.370573
TTCACTC153.8133454E-5194.370575
TGATCGC100.0029468904194.370573
TGATCCC204.925296E-7194.370577
TGTTCGC23650.0194.370573
CCTGATC100.0029468904194.370571
Produced by FastQC (version 0.11.5)